Ultrasensitive detection of interleukin 1? using 3-phosphonopropionic acid modified FTO surface as an effective platform for disposable biosensor fabrication

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dc.authorid0000-0003-3042-1087en_US
dc.authorscopusid6506764918en_US
dc.authorwosidAAY-1439-2021en_US
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2024-12-20T13:21:10Z
dc.date.available2024-12-20T13:21:10Z
dc.date.issued2021en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.description.abstractIn this study, we utilized a carboxyalkylphosphonic acid covered fluorine doped tin oxide (FTO) as an electrode material for fabrication of Interleukin 1α (IL-1α) immunosensor. For this aim, anti-IL-1α antibodies were attached on the 3-phosphonopropionic acid (PHP) modified FTO surface covalently. Electrochemical (electrochemical impedance spectroscopy and cyclic voltammetry) and morphological (scanning electron microscopy and atomic force microscopy) characterizations were performed to monitor the successful fabrication of immunoelectrodes. After incubation of anti-IL-1α antibody immobilized FTO electrodes in IL-1α antigen solutions, increases were seen in impedimetric responses. IL-1α antigen was determined in a linear detection range from 0.02 to 2 pg/mL by EIS. The detection limit of the suggested immunosensor was 6 fg/mL. The applicability of the designed biosensor was tested by using human serum and saliva samples and acceptable results were obtained. In addition, high sensitivity, good specificity, low detection limit made the proposed immunosensor a potential technique for IL-1α antigen determination in routine clinical analysis.en_US
dc.identifier.citationAydın, E. B., & Sezgintürk, M. K. (2021). Ultrasensitive detection of interleukin 1α using 3-phosphonopropionic acid modified FTO surface as an effective platform for disposable biosensor fabrication. Bioelectrochemistry, 138, 107698. https://doi.org/10.1016/j.bioelechem.2020.107698en_US
dc.identifier.doi10.1016/j.bioelechem.2020.107698
dc.identifier.issn1567-5394
dc.identifier.issn1878-562X
dc.identifier.pmid33254051
dc.identifier.scopus2-s2.0-85096860710
dc.identifier.urihttps://doi.org/10.1016/j.bioelechem.2020.107698
dc.identifier.urihttps://hdl.handle.net/20.500.12428/6771
dc.identifier.volume138en_US
dc.identifier.wosWOS:000663533800017
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevier B.V.en_US
dc.relation.ispartofBioelectrochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject3-Phosphonopropionic aciden_US
dc.subjectFluorine doped tin oxideen_US
dc.subjectImpedimetric biosensoren_US
dc.subjectInterleukin-1αen_US
dc.titleUltrasensitive detection of interleukin 1? using 3-phosphonopropionic acid modified FTO surface as an effective platform for disposable biosensor fabrication
dc.typeArticle

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