Nano-hydroxyapatite incorporated quince seed mucilage bioscaffolds for osteogenic differentiation of human adipose-derived mesenchymal stem cells

Basit Öğe Kaydı
dc.authoridÇetin Genç, Çiğdem / 0000-0001-6745-3790en_US
dc.authoridYılmaz, Hilal Deniz / 0000-0003-0412-2868en_US
dc.authoridArslan, Yavuz Emre / 0000-0003-3445-1814en_US
dc.contributor.authorÇetin Genç, Çiğdem
dc.contributor.authorYılmaz, Hilal Deniz
dc.contributor.authorKaraca, Burak
dc.contributor.authorKıran, Fadime
dc.contributor.authorArslan, Yavuz Emre
dc.date.accessioned2023-05-16T06:43:07Z
dc.date.available2023-05-16T06:43:07Z
dc.date.issued2022en_US
dc.departmentFakülteler, Diş Hekimliği Fakültesi, Ortodonti Ana Bilim Dalı
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.description.abstractIn this study, the therapeutic hydrocolloid quince seed mucilage (QSM) from Cydonia oblonga Miller fruit is enriched with needle-like nano-hydroxyapatite (nHAp) crystals to fabricate a novel biomimetic osteogenic bioscaffold. The molecular weight (Mw) of water-based extracted QSM was measured with GPC (8.67 × 105 g/mol), and the composite blend was prepared at a ratio of 1:1 (w/w) QSMaq and nHAp. The porous bioscaffolds were manufactured by the freeze-drying method, and evaluated in-depth with advanced analyses. The XRD, ATR-FTIR, SEM-EDX, and elemental mapping analyses revealed a uniform coated semi-crystalline structure with no covalent bindings between QSM and nHAp. Moreover, due to the hydrocolloid backbone, a supreme swelling ratio (w/w, 6523 ± 190%) with suitable pore size (208.12 ± 99.22 μm) for osteogenic development was obtained. Further, the cytocompatible bioscaffolds were evaluated for osteogenic differentiation in vitro using human adipose-derived mesenchymal stem cells (hAMSCs). The immuno/histochemical (I/HC) staining revealed that the cells with the spherical morphology invaded the pores of the prepared bioscaffolds. Also, relatively early up-regulated osteogenic markers were observed by the qRT-PCR analyses. Overall, it is believed that the QSM-nHAp bioscaffolds might be favorable in non-load bearing applications, especially in the cranio-maxillofacial region, due to their regenerative, bendable, and durable features.en_US
dc.identifier.citationCetin Genc, C., Yilmaz, H. D., Karaca, B., Kiran, F., & Arslan, Y. E. (2022). Nano-hydroxyapatite incorporated quince seed mucilage bioscaffolds for osteogenic differentiation of human adipose-derived mesenchymal stem cells. International Journal of Biological Macromolecules, 195, 492-505. doi:10.1016/j.ijbiomac.2021.12.054en_US
dc.identifier.doi10.1016/j.ijbiomac.2021.12.054
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid34921891
dc.identifier.scopus2-s2.0-85121357907
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2021.12.054
dc.identifier.urihttps://hdl.handle.net/20.500.12428/4167
dc.identifier.volume195en_US
dc.identifier.wosWOS:000734407000006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÇetin Genç, Çiğdem
dc.institutionauthorYılmaz, Hilal Deniz
dc.institutionauthorKaraca, Burak
dc.institutionauthorArslan, Yavuz Emre
dc.language.isoen
dc.publisherElsevier B.V.en_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBioscaffolden_US
dc.subjectBone tissue engineeringen_US
dc.subjectNano-hydroxyapatiteen_US
dc.subjectNon-load bearing bone graftsen_US
dc.subjectOsteoinductiveen_US
dc.subjectQuince seed mucilageen_US
dc.titleNano-hydroxyapatite incorporated quince seed mucilage bioscaffolds for osteogenic differentiation of human adipose-derived mesenchymal stem cells
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Cigdem_Cetin_Genc_Makale.pdf
Boyut:
23.04 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Araştırma Makalesi
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Biyomühendislik Bölümü Koleksiyonu
Ortodonti Bölümü Yayın Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu