DETERMINATION OF APPROPRIATE ENDOGENOUS REFERENCE GENES FOR RT-QPCR ANALYSIS IN SYRIAN (GOLDEN) HAMSTERS AND MONGOLIAN GERBILS

Basit Öğe Kaydı
dc.contributor.authorGunduz, Bulent
dc.contributor.authorOnder, Betul
dc.contributor.authorUzgan, Tanay
dc.date.accessioned2025-01-27T20:11:40Z
dc.date.available2025-01-27T20:11:40Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPurpose: The use of hamsters and gerbils has increased significantly in a variety of fields, including biological rhythms, reproductive biology, immunology, oncology, and many others. Material and Methods: The most stable genes in Syrian hamsters ( Mesocricetus auratus) ) and Mongolian gerbils ( Meriones unguiculatus) ) were assessed using 32 reference genes for normalization in RT-qPCR analysis. Adrenal, cerebral cortex, heart, hypothalamus, kidney, liver, lung and testis tissues were used to extract and purify RNAs. GeNorm was used to determine the gene expression stabilities of 14 candidate endogenous genes from each tissue that was compatible for both animals. Results: Under our experimental conditions, we discovered that two endogenous genes are adequate for each tissue to perform RT-qPCR normalization. There were differences in the most stable genes between species and tissues. Conclusion: We suggest that combinations of endogenous genes ought to be carefully chosen under various experimental circumstances.
dc.description.sponsorshipTUEBITAK COST (European Cooperation in Science and Technology - COST Association) [111T639]
dc.description.sponsorshipThis research was funded by TUEBITAK COST (European Cooperation in Science and Technology - COST Association) [Grant No: 111T639, 2011] .
dc.identifier.doi10.30621/jbachs.1315023
dc.identifier.endpage542
dc.identifier.issn2458-8938
dc.identifier.issn2564-7288
dc.identifier.issue3
dc.identifier.startpage533
dc.identifier.trdizinid1274267
dc.identifier.urihttps://doi.org/10.30621/jbachs.1315023
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1274267
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20657
dc.identifier.volume8
dc.identifier.wosWOS:001334630400003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherDokuz Eylul Univ Inst Health Sciences
dc.relation.ispartofJournal of Basic and Clinical Health Sciences
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectHamsters
dc.subjectgerbils
dc.subjectreference genes
dc.subjectendogenous genes
dc.subjectqPCR
dc.titleDETERMINATION OF APPROPRIATE ENDOGENOUS REFERENCE GENES FOR RT-QPCR ANALYSIS IN SYRIAN (GOLDEN) HAMSTERS AND MONGOLIAN GERBILS
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu