Intranasal miRNAs-17/20 Administration Alleviates Early Brain Injury After Subarachnoid Hemorrhage in Rats
dc.authorid | Ovali, Mehmet Akif/0000-0001-8740-6422 | |
dc.authorid | uzun, metehan/0000-0003-1406-5473 | |
dc.contributor.author | Malcok, U. A. | |
dc.contributor.author | Doganlar, O. | |
dc.contributor.author | Tufekcioglu, N. K. | |
dc.contributor.author | Ovali, M. A. | |
dc.contributor.author | Aykora, D. | |
dc.contributor.author | Doganlar, Z. B. | |
dc.contributor.author | Buyuk, B. | |
dc.date.accessioned | 2025-01-27T20:45:37Z | |
dc.date.available | 2025-01-27T20:45:37Z | |
dc.date.issued | 2023 | |
dc.department | Çanakkale Onsekiz Mart Üniversitesi | |
dc.description.abstract | Early brain injury (EBI) in the first 24-72 h is the leading cause of mortality and disability related to subarachnoid hemorrhage (SAH). Both melatonin and microRNAs (miRs) are involved in the regulation of a number of neuronal molecular signaling procedures in the central nervous system, ranging from hypoxia, inflammation to neuronal apoptosis. The present study was performed to explore the effect of miRs-17/20 and combined treatment with melatonin on early brain injury after SAH and underlying molecular mechanisms in rats. In this study 54 Wistar albino rats were divided into six experimental groups: Sham, SAH, SAH + Melatonin, SAH+miRs-17/20 control, SAH+MEL+miRs-17/20, and SAH+MEL+miRs-17/20. The Garcia's Neurological Scoring Scale and motor coordination tests were used for clinical observation. H&E staining was performed to evaluate pathological score. The gene expression levels were determined by qRT-PCR and key proteins were quantitated by Western blot assay. miRs-17/20 with or without melatonin treatment suppressed the expression and activity of both the HIF1/VEGF/MMPs and the IL6R/JAK2/STAT3 axis. miRs-17/20 with or without melatonin treatment also mitigated the clinical impairment, pyknosis, and edema in the hippocampus and cortex and neurodegeneration induced by SAH. Our results show that miRs-17/20 alleviated EBI by reducing hypoxic conditions, hypoxia-induced molecular signaling, and neuronal apoptosis. | |
dc.description.sponsorship | Scientific Research Coordination Unit (BAPD) of Canakkale Onsekiz Mart University, Canakkale, Turkey [TSA-2019-2943] | |
dc.description.sponsorship | This work was funded by the Scientific Research Coordination Unit (BAPD) of Canakkale Onsekiz Mart University, Canakkale, Turkey (Project Number: TSA-2019-2943). | |
dc.identifier.doi | 10.1007/s11094-023-02953-7 | |
dc.identifier.endpage | 808 | |
dc.identifier.issn | 0091-150X | |
dc.identifier.issn | 1573-9031 | |
dc.identifier.issue | 6 | |
dc.identifier.scopus | 2-s2.0-85174254620 | |
dc.identifier.scopusquality | Q4 | |
dc.identifier.startpage | 793 | |
dc.identifier.uri | https://doi.org/10.1007/s11094-023-02953-7 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12428/24658 | |
dc.identifier.volume | 57 | |
dc.identifier.wos | WOS:001084826300007 | |
dc.identifier.wosquality | Q4 | |
dc.indekslendigikaynak | Web of Science | |
dc.indekslendigikaynak | Scopus | |
dc.language.iso | en | |
dc.publisher | Springer | |
dc.relation.ispartof | Pharmaceutical Chemistry Journal | |
dc.relation.publicationcategory | info:eu-repo/semantics/openAccess | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.snmz | KA_WoS_20250125 | |
dc.subject | subarachnoid hemorrhage early brain injury | |
dc.subject | microRNAs-17/20 | |
dc.subject | melatonin | |
dc.title | Intranasal miRNAs-17/20 Administration Alleviates Early Brain Injury After Subarachnoid Hemorrhage in Rats | |
dc.type | Article |