Intranasal miRNAs-17/20 Administration Alleviates Early Brain Injury After Subarachnoid Hemorrhage in Rats

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dc.authoridOvali, Mehmet Akif/0000-0001-8740-6422
dc.authoriduzun, metehan/0000-0003-1406-5473
dc.contributor.authorMalcok, U. A.
dc.contributor.authorDoganlar, O.
dc.contributor.authorTufekcioglu, N. K.
dc.contributor.authorOvali, M. A.
dc.contributor.authorAykora, D.
dc.contributor.authorDoganlar, Z. B.
dc.contributor.authorBuyuk, B.
dc.date.accessioned2025-01-27T20:45:37Z
dc.date.available2025-01-27T20:45:37Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractEarly brain injury (EBI) in the first 24-72 h is the leading cause of mortality and disability related to subarachnoid hemorrhage (SAH). Both melatonin and microRNAs (miRs) are involved in the regulation of a number of neuronal molecular signaling procedures in the central nervous system, ranging from hypoxia, inflammation to neuronal apoptosis. The present study was performed to explore the effect of miRs-17/20 and combined treatment with melatonin on early brain injury after SAH and underlying molecular mechanisms in rats. In this study 54 Wistar albino rats were divided into six experimental groups: Sham, SAH, SAH + Melatonin, SAH+miRs-17/20 control, SAH+MEL+miRs-17/20, and SAH+MEL+miRs-17/20. The Garcia's Neurological Scoring Scale and motor coordination tests were used for clinical observation. H&E staining was performed to evaluate pathological score. The gene expression levels were determined by qRT-PCR and key proteins were quantitated by Western blot assay. miRs-17/20 with or without melatonin treatment suppressed the expression and activity of both the HIF1/VEGF/MMPs and the IL6R/JAK2/STAT3 axis. miRs-17/20 with or without melatonin treatment also mitigated the clinical impairment, pyknosis, and edema in the hippocampus and cortex and neurodegeneration induced by SAH. Our results show that miRs-17/20 alleviated EBI by reducing hypoxic conditions, hypoxia-induced molecular signaling, and neuronal apoptosis.
dc.description.sponsorshipScientific Research Coordination Unit (BAPD) of Canakkale Onsekiz Mart University, Canakkale, Turkey [TSA-2019-2943]
dc.description.sponsorshipThis work was funded by the Scientific Research Coordination Unit (BAPD) of Canakkale Onsekiz Mart University, Canakkale, Turkey (Project Number: TSA-2019-2943).
dc.identifier.doi10.1007/s11094-023-02953-7
dc.identifier.endpage808
dc.identifier.issn0091-150X
dc.identifier.issn1573-9031
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85174254620
dc.identifier.scopusqualityQ4
dc.identifier.startpage793
dc.identifier.urihttps://doi.org/10.1007/s11094-023-02953-7
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24658
dc.identifier.volume57
dc.identifier.wosWOS:001084826300007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPharmaceutical Chemistry Journal
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectsubarachnoid hemorrhage early brain injury
dc.subjectmicroRNAs-17/20
dc.subjectmelatonin
dc.titleIntranasal miRNAs-17/20 Administration Alleviates Early Brain Injury After Subarachnoid Hemorrhage in Rats
dc.typeArticle

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