In Vivo Wound Healing and Immune Response Studies of Chitosan Cryogels With Invertebrate Model Organism Galleria mellonella

Basit Öğe Kaydı
dc.contributor.authorEkici, Sema
dc.contributor.authorKaya, Serhat
dc.contributor.authorDurucu, Gurkan
dc.date.accessioned2026-02-03T12:03:19Z
dc.date.available2026-02-03T12:03:19Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the present study, it was aimed to prepare single and double network chitosan (Ch) cryogels cross-linked with glutaraldehyde (G), which can be recommended for use as model wound dressings and hemostatic agents, and to reveal in vivo studies with Galleria mellonella. An in vivo study about Ch cryogels with these larvae was not declared in the literature, so our study is the first of its kind. G. mellonella was used to determine the effects of cryogels on immunity, oxidative stress, and wound healing. Cinnamic acid (CA) was loaded onto the cryogels, and the percent cumulative release data of CA were found to be in the range of 69%-80%. The results show that loading of CA onto [Ch-3]cry cryogels considerably improved immune responses; the [Ch-3]cry-CA group was the most successful in terms of immunological response, oxidative stress balance, and wound healing. In accordance with the 3R principles of ethical animal research, the use of G. mellonella in this study served as a scientifically relevant and ethically responsible alternative model to mammals for preliminary assessment of wound healing potential and innate immune activation. The porous structures, high mechanical strengths, and rapidly swelling-deswelling abilities of [Ch-2@Ch]cry and [Ch-3]cry cryogels indicated that these may be suitable for biomedical applications. Analysis of SEM micrographs indicated that the morphology of dual network cryogels prepared in the form of interpenetrating polymeric networks (IPNs) was more regular and homodispersed with respect to single network cryogels. The compressive elasticity modulus (E) values of IPNs cryogels (0.160 N/mm) is approximately 4.6 times that of Ch cryogels with a single network (0.035 N/mm).
dc.description.sponsorshipScientific Research Commission of Canakkale Onsekiz Mart University [BAP FBA- 2021- 3548]
dc.description.sponsorshipThe authors gratefully acknowledge financial support provided by the Scientific Research Commission of Canakkale Onsekiz Mart University (BAP FBA- 2021- 3548).
dc.identifier.doi10.1002/bip.70042
dc.identifier.issn0006-3525
dc.identifier.issn1097-0282
dc.identifier.issue5
dc.identifier.pmid40736166
dc.identifier.scopus2-s2.0-105012221658
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/bip.70042
dc.identifier.urihttps://hdl.handle.net/20.500.12428/35018
dc.identifier.volume116
dc.identifier.wosWOS:001574387300009
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiopolymers
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260130
dc.subjectchitosan cryogel
dc.subjectfull-IPN cryogel
dc.subjectG. mellonella
dc.subjecthemostatic material
dc.subjectwound dressings
dc.titleIn Vivo Wound Healing and Immune Response Studies of Chitosan Cryogels With Invertebrate Model Organism Galleria mellonella
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu