Early detection of the ovarian cancer with a label-free and disposable ITO-PET based immunosensor platform

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dc.authoridOzcan, Burcu/0000-0002-5123-5972
dc.authoridÖzay, Özgür/0000-0001-6589-9844
dc.contributor.authorOzcan, Burcu
dc.contributor.authorOzay, Hava
dc.contributor.authorÖzay, Özgür
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T21:13:08Z
dc.date.available2025-01-27T21:13:08Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractAlpha fetoprotein (AFP), a popular biomarker for ovarian cancer, was the target of this study to create an immunosensor based on electrochemical impedance spectroscopy (EIS). For this purpose, a disposable Indium tin oxide coated PET (In2O3/SnO2, ITO-PET) electrode was used as a basic electrode for working of the triple electrode system and the electrodes were modified with 3-(Trimethoxy silyl) propyl methacrylate (3- TMSPM) silanization agent. Although there are studies on the determination of AFP, the determination of AFP with a cheap, flexible and sensitive electrode system such as ITO-PET in this study makes the study remarkable. With the designed biosensors, AFP protein determination at a wide concentration range (0.05 pg/mL-100 pg/mL) could be successfully performed. To prove that the designed immunosensor was constructed correctly, linear range, repeatability, reproducibility and regeneration capacity (characterization studies) were investigated. Obtaining successful results in the storage capacity studies of the biosensor, which has high reproducibility and repeatability, showed that it also has potential for commercial use. The high specificity of the biosensor also proves its reliability. Finally, the performance of the biosensor in commercial human serum samples was also evaluated.
dc.description.sponsorshipBAP at Canakkale Onsekiz Mart University [FBA-2020-3436]
dc.description.sponsorshipfor this study was graciously supplied by BAP at Canakkale Onsekiz Mart University (Project Number: FBA-2020-3436).
dc.identifier.doi10.1016/j.microc.2024.111340
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.scopus2-s2.0-85200408428
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.microc.2024.111340
dc.identifier.urihttps://hdl.handle.net/20.500.12428/28304
dc.identifier.volume205
dc.identifier.wosWOS:001288283600001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMicrochemical Journal
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBiosensor
dc.subject3-(Trimethoxy silyl)propyl methacrylate
dc.subjectAlpha fetoprotein
dc.subjectOvarian cancer
dc.titleEarly detection of the ovarian cancer with a label-free and disposable ITO-PET based immunosensor platform
dc.typeArticle

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