Electrochemical oxidation of prednisolone at glassy carbon electrode and its quantitative determination in human serum and tablets by Osteryoung square wave voltammetry
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Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bentham Science Publ Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A simple, rapid, selective and sensitive electrochemical method for the direct determination of prednisolone in spiked human serum and tablets was developed. The electrochemical oxidation and determination of prednisolone has been carried out at glasy carbon electrode (GCE) in various aquaeous solution in the pH range of 0.56-12.30 by cyclic (CV) and Osteryoung square wave voltammetry (OSWV). The best results were obtained for the determination of prednisolone by OSWV method in 0.5 mol L-1 sulphuric acid at about pH 0.56. The peak current and peak potential depends on pH, so its influence and also scan rate were studied. The diffusion controlled by nature of the peak was established. This electroanalytical procedure enabled to determine prednisolone in the concentration range 1.0 X 10(-6)- 2.0 x 10(-5) mol L-1. This limit of detection (LOD) and limit of quantification (LOQ) were obtained as 3.4x10(-7) and 4.5x 10(-7) mol L-1 respectively. Precision and accuracy of the developed method were checked by recovery studies in spiked tablets and human serum.
Açıklama
Anahtar Kelimeler
prednisolone, voltammetry, determination, tablet and serum
Kaynak
Current Analytical Chemistry
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
3
Sayı
1
