An ultrasensitive electrochemical immunosensor platform based on disposable ITO electrode modified by 3-CPTMS for early detection of parathyroid hormone

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dc.authoridUludag, Inci/0000-0003-4296-2657
dc.contributor.authorUludag, Inci
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T21:04:21Z
dc.date.available2025-01-27T21:04:21Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractParathyroid hormone (PTH) is a polypeptide containing 84 amino acids secreted by cells of the parathyroid glands. Imbalances of parathyroid levels cause medical problems such as osteoporosis, mental disorders, pancreatitis, kidney stones, cancer, and other symptoms. In this study, we aimed to design an ultrasensitive electrochemical immunosensor for PTH detection. Indium tin oxide (ITO) was used as an electrode for electrochemical impedance spectroscopy (EIS) measurements. ITO sheets were modified by using 3-cyanopropyltrimethoxysilane (3-CPTMS) self-assembled monolayers (SAMs) for immobilizing the anti-PTH antibody via covalent interactions. Cyclic voltammetry (CV) and EIS methods were applied to characterize immobilization steps. Therefore, 1% was selected for an optimal concentration of silane, 10 ng/mL was selected as an optimal concentration of anti-PTH, and 30 and 45 min were selected as optimal incubation times for anti-PTH and PTH, respectively. PTH antigen was determined in the concentration range from 0.05 fg/mL to 150 fg/mL. To detect the analytical characterization of the 3-CPTMS modified immunosensor, linear range, repeatability, reproducibility, Kramers-Kronig transform, and regeneration studies were performed. Also, the shelf life of the developed biosensor was investigated. Finally, real human serum samples were analyzed with the PTH immunosensor. The results showed that the designed biosensor system has high potential for early detection for medical treatments.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [113 Z 678]
dc.description.sponsorshipWe are grateful for the financial support of this research by the Scientific and Technological Research Council of Turkey (TUBITAK Project Number: 113 Z 678).
dc.identifier.doi10.3906/kim-1909-44
dc.identifier.endpage1710
dc.identifier.issn1300-0527
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85077197307
dc.identifier.scopusqualityQ3
dc.identifier.startpage1697
dc.identifier.trdizinid336126
dc.identifier.urihttps://doi.org/10.3906/kim-1909-44
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/336126
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27620
dc.identifier.volume43
dc.identifier.wosWOS:000501598500015
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Chemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBiosensor
dc.subjectdisposable immunosensor
dc.subjectITO-PET electrode
dc.subjectparathyroid hormone
dc.subject3-CPTMS
dc.titleAn ultrasensitive electrochemical immunosensor platform based on disposable ITO electrode modified by 3-CPTMS for early detection of parathyroid hormone
dc.typeArticle

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