Transformation of Cytotoxic Linear Polyethyleneimine (L-PEI) Into Biocompatible and Hemacompatible Crosslinked Particles With Antimicrobial Properties

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dc.contributor.authorDemirci, Sahin
dc.contributor.authorSuner, Selin S.
dc.contributor.authorSahiner, Mehtap
dc.contributor.authorGuven, Olgun
dc.contributor.authorSahiner, Nurettin
dc.date.accessioned2026-02-03T12:03:19Z
dc.date.available2026-02-03T12:03:19Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractLinear polyethyleneimine (L-PEI) microgel/particles were prepared using glycerol diglycidyl ether (GDE) as crosslinker at various mole ratios, 10%, 25%, and 50% of L-PEI repeating unit, and designated as L-PEI-1, L-PEI-2, and L-PEI-3, respectively. All microgels were found blood compatible with < 2% hemolysis and > 80% blood clotting indexes at 1000 mu g/mL concentrations, whereas L-PEI is hemolytic (> 80% hemolysis). Similarly, L-PEI showed cytotoxicity on L929 fibroblasts even at 50 mu g/mL, while > 80% cell viability was observed for L-PEI-based particles even at 1000 mu g/mL. Although it is regarded as less cytotoxic than branched PEI, the cross-linked L-PEI particles effectively circumvent cytotoxicity as the most important limitation of L-PEI in biological applications. Additionally, the higher antimicrobial activity against Escherichia coli, gram-negative bacteria, Bacillus subtilis, gram-positive bacteria strains, and Candida albicans, yeast strains was observed for L-PEI-1 and protonated L-PEI-1 particles. Moreover, the antibacterial activity of L-PEI-1 particles was further improved after the inclusion of Ag(I) and Cu(II) ions within L-PEI particles as Ag@L-PEI-1 and Cu@L-PEI-1 composites, respectively. The lower MIC and MBC values were determined for Ag@L-PEI-1 particles against Escherichia coli and Bacillus subtilis as 0.079 and 0.312 mg/mL, respectively, and an MFC value for Candida albicans was 0.156 mg/mL for Ag@L-PEI-1 particles.
dc.identifier.doi10.1002/app.70209
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.scopus2-s2.0-105025558934
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/app.70209
dc.identifier.urihttps://hdl.handle.net/20.500.12428/35021
dc.identifier.wosWOS:001644336600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260130
dc.subjectbiomedical applications
dc.subjectcrosslinking
dc.subjectfunctionalization of polymers
dc.subjecthydrophilic polymers
dc.subjectstimuli-sensitive polymers
dc.titleTransformation of Cytotoxic Linear Polyethyleneimine (L-PEI) Into Biocompatible and Hemacompatible Crosslinked Particles With Antimicrobial Properties
dc.typeArticle

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