Investigation of Stem Cell Applications on In Vitro Fertilization in Rats

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dc.authorid0000-0002-9435-8139en_US
dc.authorscopusid57193693689en_US
dc.authorwosidFOZ-5231-2022en_US
dc.contributor.authorGümürdü, A.
dc.contributor.authorÖztürk, Şamil
dc.contributor.authorAydemir, I.
dc.contributor.authorTuğlu, M. İ.
dc.date.accessioned2022-12-31T09:30:54Z
dc.date.available2022-12-31T09:30:54Z
dc.date.issued2021en_US
dc.departmentMeslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü
dc.description.abstractWe aimed to search the effects of bone marrow-derived mesenchymal stem cell-conditioned media on in vitro fertilization by investigation of lifetime of germ cells cleavage, degeneration rates and embryo quality. For this purpose, firstly MSCs were isolated from femurs and tibias of the rat, and cells were cultured until the fourth passage. Sperm and oocytes were collected from male and female rats. Oocytes were added in Human Tubal Fluid Media (HTFM), Single Step Media (SSM), Alpha-MEM Media (AMM) and Bone Marrow-Derived Mesenchymal Stem Cell-Conditioned Media (CM). Thousand sperm were added into the media which including oocytes. Embryos were allowed to produce by IVF. The development of the embryos was followed until the 11th day, and the arrest, degeneration rates and alive embryos were established. The embryos reached 2, 4, 8, 16 cells stages and morula stage in the CM. While AMM had a negative effect on fertilization and embryo development, the most favourable effect was shown to be caused by CM in comparison with the other medias. These results have shown that the beneficial effects of CM in IVF would be a significant increase in the rate of fertility and development of embryosen_US
dc.identifier.citationGumurdu, A., Ozturk, S., Aydemir, I., & Tuglu, M. I. (2021). Investigation of stem cell applications on in vitro fertilization in rats. Journal of the Hellenic Veterinary Medical Society, 72(3), 3067-3074. doi:10.12681/JHVMS.28489en_US
dc.identifier.doi10.12681/JHVMS.28489
dc.identifier.endpage3074en_US
dc.identifier.issn1792-2720
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85119664175
dc.identifier.startpage3067en_US
dc.identifier.urihttps://doi.org/10.12681/jhvms.28489
dc.identifier.urihttps://hdl.handle.net/20.500.12428/3763
dc.identifier.volume72en_US
dc.identifier.wosWOS:000713498000009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorÖztürk, Şamil
dc.language.isoen
dc.publisherHELLENIC VETERINARY MEDICAL SOC.en_US
dc.relation.ispartofJournal of the Hellenic Veterinary Medical Societyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBone Marrow Mesenchymal Stem Cellen_US
dc.subjectIn Vitro Fertilizationen_US
dc.subjectOocyteen_US
dc.subjectSpermen_US
dc.subjectSuperovulationen_US
dc.titleInvestigation of Stem Cell Applications on In Vitro Fertilization in Rats
dc.typeArticle

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