Genetic variability in the prion protein gene in five indigenous Turkish sheep breeds

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dc.authoridUzun, Metehan / 0000-0003-1406-5473
dc.authoridGutierrez-Gil, Beatriz/0000-0001-7990-5723
dc.authorid/0000-0001-9058-131X
dc.contributor.authorAlvarez, Lorena
dc.contributor.authorGutierrez-Gil, Beatriz
dc.contributor.authorUzun, Metehan
dc.contributor.authorSan Primitivo, Fermin
dc.contributor.authorJose Arranz, Juan
dc.date.accessioned2025-01-28T21:24:05Z
dc.date.available2025-01-28T21:24:05Z
dc.date.issued2011
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractOvine susceptibility to scrapie is largely controlled by polymorphisms in the PRNP gene. Beginning in 2003, breeding programmes based on the known association between this gene and the susceptibility/resistance status of this disease have been implemented in many European countries. This is not the case in Turkey, where the PRNP gene was not genotyped in native sheep breeds until recently. We sequenced the complete open reading frame of the PRNP gene in 100 sheep belonging to five native Turkish sheep breeds (Akkaraman = 21; Morkaraman = 20; Tuj = 17; Hemsin = 23; Karayaka = 19). Based on the variability found at codons 136, 154 and 171 (also referred to as standard codons), we determined six alleles VRQ TRQ ARR, ARH, ARK and ARQ. The archetype, ARQ was the most frequent variant in each of the five breeds (across-breed frequency = 0.710), while the second most frequent was the resistance-associated ARR allele (0.140). The susceptible VRQ allele exhibited the lowest frequency (0.015). The AHQ allele was not found in any of the analysed breeds. Beyond variability in the standard codons, we found ten additional amino acid variants (M112T, G127S, G127V, M137T, L141F, H143R, N146S, Y172D, Q189L and V213E), one of which, V213E, has not been previously described to our knowledge. Overall, this report may serve as a complement of previous studies on the genetic background of native Turkish sheep regarding the resistance/susceptibility status to classical and atypical scrapie. (C) 2011 Elsevier B.V. All rights reserved.
dc.description.sponsorshipCastilla and Leon regional government (Junta de Castilla y Leon, Spain); State Planning Organisation (DPT) of Turkey; EU trough European Commission [030278, FPC-2004-SMW-COLL]; Spanish Ministry of Science; [GR43]
dc.description.sponsorshipThis work was supported by Castilla and Leon regional government (Junta de Castilla y Leon, Spain) by a grant for research groups of excellence (Project GR43) by the State Planning Organisation (DPT) of Turkey. Financial support of EU trough the Sixth Framework Program of the European Commission, project RISKSCRA, contract no 030278, FPC-2004-SMW-COLL is also acknowledged. Beatriz Gutierrez-Gil is funded by the Juan de la Cierva Program of the Spanish Ministry of Science.
dc.identifier.doi10.1016/j.smallrumres.2011.03.043
dc.identifier.endpage98
dc.identifier.issn0921-4488
dc.identifier.issn1879-0941
dc.identifier.issue2-3
dc.identifier.scopus2-s2.0-79961210468
dc.identifier.scopusqualityQ2
dc.identifier.startpage93
dc.identifier.urihttps://doi.org/10.1016/j.smallrumres.2011.03.043
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26412
dc.identifier.volume99
dc.identifier.wosWOS:000294525300003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorUzun, Metehan
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofSmall Ruminant Research
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectScrapie
dc.subjectVariability
dc.subjectPRNP gene
dc.subjectSheep
dc.subjectTurkish breeds
dc.titleGenetic variability in the prion protein gene in five indigenous Turkish sheep breeds
dc.typeArticle

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