Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

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dc.authorid0000-0002-5123-5972en_US
dc.authorid0000-0003-3042-1087en_US
dc.authorscopusid56106034900en_US
dc.authorscopusid6506764918en_US
dc.authorwosidAAY-1451-2021en_US
dc.authorwosidAAY-1439-2021en_US
dc.contributor.authorÖzcan, Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2023-05-10T07:45:13Z
dc.date.available2023-05-10T07:45:13Z
dc.date.issued2021en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.description.abstractThis study presents a disposable, novel, and sensitive biosensing system to determine adiponectin, an obesity biomarker, in real human serum. The graphite paper (GP) working electrode is a new material for impedimetric biosensors. In the literature, there is no study in which this electrode is used in impedance-based biosensors for adiponectin detection. Sensitive and useful techniques, such as electrochemical impedance spectroscopy and cyclic voltammetry, were utilized for investigation of the modification of the GP electrode surface and optimization and characterization of the constructed biosensor. The single frequency impedance technique was used to study the interactions between antiadiponectin and adiponectin. The morphology of the electrode surface for each immobilization step was examined with scanning electron microscopy. All experimental parameters were optimized to fabricate a rapid and sensitive biosensing system. The designed biosensor presents excellent performance with a wide detection range (0.05-25 pg mL-1) and a low limit of detection (0.0033 pg mL-1) for adiponectin determination. Also, it has been demonstrated that the biosensor sensitively allows for the detection of adiponectin in human serum. The affinity of the designed immunosensor toward other proteins and components was examined in the presence of the target protein (adiponectin), leptin (100 pg mL-1), creatine kinase (50 pg mL-1), parathyroid hormone (50 pg mL-1), and d-glucose (0.5 M). The selectivity of the adiponectin biosensor resulted in high capacity to neglect the interference effect. The constructed biosensor showed good linearity, long-Term storage life (10 weeks), high reusability (18 times regenerability), and high ability to detect adiponectin concentrations at picogram levels. ©en_US
dc.identifier.citationOzcan, B., & Sezginturk, M. K. (2021). Highly sensitive and single-use biosensing system based on a GP electrode for analysis of adiponectin, an obesity biomarker. ACS Biomaterials Science and Engineering, 7(8), 3658-3668. doi:10.1021/acsbiomaterials.1c00094en_US
dc.identifier.doi10.1021/acsbiomaterials.1c00094
dc.identifier.endpage3668en_US
dc.identifier.issn2373-9878
dc.identifier.issue8en_US
dc.identifier.pmid34260213
dc.identifier.scopus2-s2.0-85111302542
dc.identifier.startpage3658en_US
dc.identifier.urihttps://doi.org/10.1021/acsbiomaterials.1c00094
dc.identifier.urihttps://hdl.handle.net/20.500.12428/4093
dc.identifier.volume7en_US
dc.identifier.wosWOS:000685089500013
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzcan, Burcu
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofACS Biomaterials Science and Engineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/113Z678
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdiponectinen_US
dc.subjectBiosensoren_US
dc.subjectChildhood obesityen_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.titleHighly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker
dc.typeArticle

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