Rapid Pathogen Purge by Photosensitive Arginine-Riboflavin Carbon Dots without Toxicity
Yükleniyor...
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mdpi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Photo-activatable antipathogenic carbon dots (CDs) were prepared by carbonization of citric acid and arginine (Arg) via 3 min microwave treatment for use in the eradication of common microorganisms. Nitrogen-doped Arg CDs were spherical in shape with a size range of 0.5 to 5 nm. The Arg CDs were modified with fluorescent dyes, such as fluorescein sodium salt (FSS, as Arg-FSS) and riboflavin (RBF, as Arg-RBF), to improve antimicrobial potency by enhancing their application in photodynamic therapy. The modified Arg CDs afforded fluorescence emission properties at 520 nm in the green region in addition to excellent blue fluorescence intensity at 420 nm under 345 nm excitation upon their FSS and RBF conjugation, respectively. Although the cytotoxicity of Arg CDs was decreased for Arg-RBF CDs to 91.2 +/- 0.7% cell viability for fibroblasts, the Arg-based CDs could be safely used for intravenous applications at 1000 mu g/mL concentration. The Arg CDs showed broad-spectrum antimicrobial activity against common pathogens and the minimum inhibitory concentration of Arg CDs was almost two-fold decreased for the modified forms without UV light. However, faster and more effective antibacterial activity was determined for photosensitive Arg-RBF CDs, with total bacterial eradication upon UV-A light exposure for 30 min.
Açıklama
Anahtar Kelimeler
dual-fluorescence carbon dots (CQ dots), arginine amino acid-derived CQ dots, fluorescein sodium salt/riboflavin dye, antimicrobial, photodynamic therapy, biocompatible CQ dots
Kaynak
Materials
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
16
Sayı
19
