A cost-effective and sensitive Au/6-mercaptohexanol/CNBr modified immunosensor approach for adiponectin analysis

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dc.contributor.authorOzcan, Burcu
dc.date.accessioned2025-01-27T20:47:51Z
dc.date.available2025-01-27T20:47:51Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThis research aimed to develop a method for analyzing adiponectin using a label-free impedimetric indium tin oxide-polyethylene terephthalate (ITO-PET) immunosensing system. A simple and stable process to design a bioelectrode modified with gold (Au)/6-mercaptohexanol (6-MH)/cyanogen bromide (CNBr)/anti-adiponectin and its biocompatibility with adiponectin was investigated. The immunosensor performance, optimization tests, and immobilization steps were all monitored and analyzed using Electrochemical Impedance spectroscopy (EIS) and cyclic voltammetry (CV). Scanning Electron microscopy (SEM) examined the surface morphology of this biosensor during its immobilization process. Testing the developed biosensor with real human serum samples allowed for an evaluation of its clinical performance. The proposed immunosensor demonstrated high repeatability, reproducibility (RSD%: 4.37 %), long storage stability, and reusability (preserving 85 % of activity in the third regeneration step), along with a low limit of detection for adiponectin antigen and a wide range of linear values. The sensor suggested a linear detection range of 0.25 pg/mL to 500 pg/mL. The limit of detection (LOD) was found to be 0.20 pg/mL, and the limit of quantification (LOQ) was determined to be 0.66 pg/mL. It appears that the biosensor decorated with Au/6-MH/CNBr could be a good choice for the selective determination of adiponectin protein, according to the statistical data.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)
dc.description.sponsorshipThe funding for this study was graciously supplied by TUBITAK (The Scientific and Technological Research Council of Turkey) .
dc.identifier.doi10.1016/j.microc.2024.112221
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.scopus2-s2.0-85209676720
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.microc.2024.112221
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25066
dc.identifier.volume207
dc.identifier.wosWOS:001363703900001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMicrochemical Journal
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBiosensor
dc.subjectAdiponectin
dc.subjectChildhood obesity
dc.titleA cost-effective and sensitive Au/6-mercaptohexanol/CNBr modified immunosensor approach for adiponectin analysis
dc.typeArticle

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