Comparison of Spectral and Molecular Analyses for Classification of Long Term Stored Wheat Samples

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dc.contributor.authorKahrıman, Fatih
dc.contributor.authorEgesel, Cem Omer
dc.date.accessioned2025-01-27T21:00:10Z
dc.date.available2025-01-27T21:00:10Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThis study aimed to determine whether NIR spectroscopy and protein band analysis can differentiate the grain samples of 15 wheat genotypes stored for different periods: Group I (91 weeks), Group II (143 weeks), Group III (194 weeks), and Group IV (246 weeks). Samples were harvested from previously-conducted field trials, and stored at +4 degrees C. A-PAGE and SDS-PAGE methods were utilized to separate gliadin and glutenin fractions, respectively. A qualitative calibration model based on the Support Vector Machine (SVM) method was generated and validated using NIR spectra taken from samples. Results indicated storage length did not have an effect on molecular band fractions. Use of this method would not be considered an effective tool for discrimination of samples stored for different lengths of time. Spectral techniques may have potential in sorting samples based on their storage time. The SVM calibration model generated here had an acceptable true classification rate (over 80%) for separating all groups, while only Groups II and IV were precisely separated (100% true classification rate) in the validation step.
dc.identifier.doi10.3964/j.issn.1000-0593(2016)04-1266-07
dc.identifier.endpage1272
dc.identifier.issn1000-0593
dc.identifier.issue4
dc.identifier.pmid30052360
dc.identifier.scopus2-s2.0-84965160763
dc.identifier.scopusqualityQ4
dc.identifier.startpage1266
dc.identifier.urihttps://doi.org/10.3964/j.issn.1000-0593(2016)04-1266-07
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26954
dc.identifier.volume36
dc.identifier.wosWOS:000374625000067
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isozh
dc.publisherOffice Spectroscopy & Spectral Analysis
dc.relation.ispartofSpectroscopy and Spectral Analysis
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectStorage
dc.subjectTriticum aestivum
dc.subjectNear infrared spectroscopy
dc.subjectGliadin
dc.subjectGlutenin
dc.titleComparison of Spectral and Molecular Analyses for Classification of Long Term Stored Wheat Samples
dc.typeArticle

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