Comparison of Spectral and Molecular Analyses for Classification of Long Term Stored Wheat Samples
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Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Office Spectroscopy & Spectral Analysis
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study aimed to determine whether NIR spectroscopy and protein band analysis can differentiate the grain samples of 15 wheat genotypes stored for different periods: Group I (91 weeks), Group II (143 weeks), Group III (194 weeks), and Group IV (246 weeks). Samples were harvested from previously-conducted field trials, and stored at +4 degrees C. A-PAGE and SDS-PAGE methods were utilized to separate gliadin and glutenin fractions, respectively. A qualitative calibration model based on the Support Vector Machine (SVM) method was generated and validated using NIR spectra taken from samples. Results indicated storage length did not have an effect on molecular band fractions. Use of this method would not be considered an effective tool for discrimination of samples stored for different lengths of time. Spectral techniques may have potential in sorting samples based on their storage time. The SVM calibration model generated here had an acceptable true classification rate (over 80%) for separating all groups, while only Groups II and IV were precisely separated (100% true classification rate) in the validation step.
Açıklama
Anahtar Kelimeler
Storage, Triticum aestivum, Near infrared spectroscopy, Gliadin, Glutenin
Kaynak
Spectroscopy and Spectral Analysis
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
36
Sayı
4
