Fabrication of Biodegradable Poly(naringin) Particles with Antioxidant Activity and Low Toxicity

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dc.authoridŞahiner, Mehtap / 0000-0001-8666-7954
dc.authoridSağbaş Suner, Selin / 0000-0002-3524-0675
dc.authoridŞahiner, Nurettin / 0000-0003-0120-530X
dc.contributor.authorŞahiner, Mehtap
dc.contributor.authorŞahiner, Nurettin
dc.contributor.authorSağbaş, Selin
dc.contributor.authorFullerton, Mitchell L.
dc.contributor.authorBlake, Diane A.
dc.date.accessioned2025-01-27T20:50:25Z
dc.date.available2025-01-27T20:50:25Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractNaringin (NR, 4', 5,7 trihydrocyflavanone-7-O-rhamnoglucoside) is a flavanone found in citrus fruit that is composed of a phenolic compound, naringenin, and a disaccharide, neohesperidose. Poly(NR) [p(NR)] particles in the size range of few micrometers to few hundred nanometers were prepared from highly purified NR and subsequently characterized using UV/visible spectroscopy, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, scanning electron microscopy, thermogravimetric analysis, and zeta potential measurements. The hydrolytic degradation of p(NR) particles at 37.5 degrees C was investigated at pHs 5.4, 7.4, and 9.0. The particles degraded most rapidly at pH 7.4, with >90% degradation after 5 h. The cytotoxicity was assessed by growing COS-1 fibroblasts for 5 days in the presence of increasing concentrations of NR or eluates from the p(NR) particles. Both NR and p(NR) particles were nontoxic for these mammalian fibroblasts; at the highest concentration tested (571 mu g/mL), the percentages of surviving cells after 5 days in culture were 96.7 +/- 0.93 and 91 +/- 11% for NR and p(NR), respectively. The effect of p(NR) on red blood cell hemolysis was also negligible (<2%) at concentrations up to 100 mu g/mL; unpolymerized NR showed slightly higher levels of hemolysis than the p(NR) particles, but values never exceeded 5% at 50 and 100 mu g/mL. Both NR and p(NR) stimulated blood coagulation in a dose-dependent manner; the hemostatic effect was greater for p(NR), which, at 100 mu g/mL, stimulated in vitro blood clotting by similar to 50%. Neither NR nor eluates from p(NR) particles inhibited alpha-glucosidase activity; in fact, both provided a modest (10-30%) stimulation at concentrations from 0.67 to 1.67 mg/mL. p(NR) particles are easily synthesized, break down readily at physiological pH, and have excellent blood and biocompatibility. As such, they will be a useful carrier for drug delivery and as an oral antioxidant supplement.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [BIDEB-2219, 1059B191600622]; Tulane University School of Medicine
dc.description.sponsorshipM.S. was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) BIDEB-2219 Postdoctoral Research program grant number 1059B191600622. Additional research funds were provided by a Research Enhancement Grant from Tulane University School of Medicine to D.A.B.
dc.identifier.doi10.1021/acsomega.8b02292
dc.identifier.endpage17367
dc.identifier.issn2470-1343
dc.identifier.issue12
dc.identifier.scopus2-s2.0-85058620180
dc.identifier.scopusqualityQ1
dc.identifier.startpage17359
dc.identifier.urihttps://doi.org/10.1021/acsomega.8b02292
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25490
dc.identifier.volume3
dc.identifier.wosWOS:000454244600197
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Omega
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectP-Glycoprotein
dc.subjectGrapefruit Juice
dc.subjectControlled-Release
dc.subjectSignaling Pathway
dc.subjectCitrus Flavonoids
dc.subjectOxidative Stress
dc.subjectNaringin
dc.subjectInhibition
dc.subjectBioavailability
dc.subjectNanoparticles
dc.titleFabrication of Biodegradable Poly(naringin) Particles with Antioxidant Activity and Low Toxicity
dc.typeArticle

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