A label-free dual immunosensor for the simultaneous electrochemical determination of CA125 and HE4 biomarkers for the early diagnosis of ovarian cancer

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dc.authorid0000-0003-3042-1087en_US
dc.authorscopusid6506764918en_US
dc.authorwosidAAY-1439-2021en_US
dc.contributor.authorBilgi Kamaç, Melike
dc.contributor.authorAltun, Muhammed
dc.contributor.authorYılmaz, Merve
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2023-08-21T08:16:08Z
dc.date.available2023-08-21T08:16:08Z
dc.date.issued2023en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.description.abstractThe blood levels of cancer antigen 125 (CA125) and human epididymal secretory protein 4 (HE4) are measured in the diagnosis and progression monitoring of ovarian cancer (OC), and the Risk of Ovarian Malignancy Algorithm (ROMA) score% values are calculated for cancer risk assessment. For the first time, disposable dual screen-printed carbon electrodes modified with reduced graphene oxide, polythionine, and gold nanoparticles were used to fabricate label-free electrochemical dual CA125-HE4 immunosensors for the sensitive, fast, and practical simultaneous determination of CA125 and HE4. DPV and SWV methods were used to simultaneously determine antigens in two different linear ranges (1–100 pg mL−1 and 1–50 ng mL−1). High sensitivity, low LOD, and LOQ were obtained for two linear ranges with a correlation coefficient above 0.99. The application stability of the dual CA125-HE4 immunosensors was determined as 60 days, and the storage stability was determined as 16 weeks. The dual immunosensors exhibited high selectivity in eight different antigen mixtures. The reusability of the dual immunosensors has been tested up to 9 cycles. ROMA score% values for pre-menopausal and post-menopausal status were calculated using the concentration of CA125 and HE4 in the blood serum and assessing OC risk. The disposable dual immunosensors can be used in point-of-care tests for rapid and practical simultaneous determination of CA125 and HE4 with high selectivity, sensitivity, and repeatability.en_US
dc.identifier.citationBilgi Kamaç, M., Altun, M., Yilmaz, M., & Sezgintürk, M.K. A label-free dual immunosensor for the simultaneous electrochemical determination of CA125 and HE4 biomarkers for the early diagnosis of ovarian cancer. Analytical and Bioanalytical Chemistry, 415(9), 1709–1718 (2023). https://doi.org/10.1007/s00216-023-04569-yen_US
dc.identifier.doi10.1007/s00216-023-04569-y
dc.identifier.endpage1718en_US
dc.identifier.issn1618-2642
dc.identifier.issn1618-2650
dc.identifier.issue9en_US
dc.identifier.pmid36719438
dc.identifier.scopus2-s2.0-85147098916
dc.identifier.startpage1709en_US
dc.identifier.urihttps://doi.org/10.1007/s00216-023-04569-y
dc.identifier.urihttps://hdl.handle.net/20.500.12428/4503
dc.identifier.volume45en_US
dc.identifier.wosWOS:000924217600003
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherSpringer Science and Business Media Deutschland GmbHen_US
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCA125en_US
dc.subjectDual electrochemical immunosensorsen_US
dc.subjectHE4en_US
dc.subjectOvarian canceren_US
dc.subjectSimultaneous determinationen_US
dc.titleA label-free dual immunosensor for the simultaneous electrochemical determination of CA125 and HE4 biomarkers for the early diagnosis of ovarian cancer
dc.typeArticle

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