A promising disposable biosensor for diagnosis of Rheumatoid Arthritis disease

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dc.authoridSezgintürk, Mustafa Kemal / 0000-0003-3042-1087
dc.authoridUzman, Ecem / 0009-0007-3714-2589
dc.contributor.authorUzman, Ecem
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-05-29T02:54:03Z
dc.date.available2025-05-29T02:54:03Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractRheumatoid Arthritis (RA) is a chronic autoimmune inflammatory disease that causes joint damage. Recently, Anti-citrullinated protein antibodies (ACPA) have been accepted as biomarkers for the diagnosis of RA due to their high specificity. This study aims to develop an immunosensor for the detection of mutated citrullinated vimentin antibody (MCV), which is autoantibody in the pathophysiology of RA from the ACPA family. For this purpose, (3-Aminopropil) trietoksisilan (3-APTES) silane agent was used to form a self-assembled monolayer (SAM) on the indium tin oxide coated polyethylene terephthalate (ITO-PET) electrode due to its sensitivity, practicality and ease of use. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measurement techniques were used in the immobilization, optimization and characterization stages and real commercial serum samples were tested for the reliability of the immunosensor. This analysis method is the first biosensor based system for the determination of MCV. By the developed immunosensor, the determination range was obtained as 0.01 pg/mL -50 pg/mL MCV with a LOD value of 7 × 10–4 pg/mL MCV. As a result, a reproducible, specific and sensitive immunosensor was fabricated. © Qatar University and Springer Nature Switzerland AG 2024.
dc.description.sponsorshipScientific and Technological Research Council of Çanakkale Onsekiz Mart University, (FYL-2022-4004)
dc.identifier.doi10.1007/s42247-024-00856-1
dc.identifier.endpage1100
dc.identifier.issn2522-5731
dc.identifier.issue2
dc.identifier.scopus2-s2.0-105003593850
dc.identifier.scopusqualityQ2
dc.identifier.startpage1087
dc.identifier.urihttps://doi.org/10.1007/s42247-024-00856-1
dc.identifier.urihttps://hdl.handle.net/20.500.12428/29902
dc.identifier.volume8
dc.identifier.wosWOS:001317342500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.ispartofEmergent Materials
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20250529
dc.subjectAnti-citrullinated protein
dc.subjectImmunosensor
dc.subjectITO-PET
dc.subjectMutated Citrulline Vimentin antibody
dc.subjectRheumatoid Arthritis
dc.titleA promising disposable biosensor for diagnosis of Rheumatoid Arthritis disease
dc.typeArticle

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