Sequence-related amplified polymorphism and inter-simple sequence repeat marker-based genetic diversity and nuclear DNA content variation in common vetch (Vicia sativa L.)

Basit Öğe Kaydı
dc.authoridTiryaki, iskender / 0000-0002-7504-2892
dc.contributor.authorÇil, Abdullah
dc.contributor.authorTiryaki, İskender
dc.date.accessioned2025-01-27T20:31:56Z
dc.date.available2025-01-27T20:31:56Z
dc.date.issued2016
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractGenetic diversity of 30 common vetch (Vicia sativa L.) lines and cultivars obtained from various resources or collected from natural flora of Turkey was evaluated by using 55 sequence-related amplified polymorphism (SRAP) and five inter-simple sequence repeat (ISSR) primer sets, and their nuclear DNA contents were determined by flow cytometer. A total of 188 polymorphic loci were detected, with an average of 3.62 loci per primer. The percentage of polymorphic loci was 82.1%. The polymorphism information content values ranged from 0.12 to 0.96, with an average of 0.63. The genetic distance coefficients were in the range of 0.112-0.627. Cluster analysis revealed that the 27 lines and three cultivars could be divided into two main groups. No polyploidy was detected within any vetch lines tested while significant (P<0.0001) nuclear DNA content differences were determined. The present study revealed that fast and accurate fingerprinting analysis could be done using SRAP and ISSR markers, which indicated existing significant variation among common vetch lines and cultivars.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK, TOVAG) [107O012]; Kahramanmaras Sutcu Imam University (KSU, BAP) [2010/4-6D]
dc.description.sponsorshipWe are grateful to The Scientific and Technological Research Council of Turkey (TUBITAK, TOVAG Grant no. 107O012) and Kahramanmaras Sutcu Imam University (KSU, BAP Grant no. 2010/4-6D) for providing financial support. We acknowledge Kahramanmaras Agricultural Research Institute, Kahramanmaras, Turkey, for providing field facilities. We thank Metin Tuna for his help on genomic DNA content determination and Ahmet Ilcim for taxonomic classification of the lines collected from natural flora of Turkey. The authors declare that they have no conflict of interest.
dc.identifier.doi10.1017/S1479262115000210
dc.identifier.endpage191
dc.identifier.issn1479-2621
dc.identifier.issn1479-263X
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84979752247
dc.identifier.scopusqualityQ2
dc.identifier.startpage183
dc.identifier.urihttps://doi.org/10.1017/S1479262115000210
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23308
dc.identifier.volume14
dc.identifier.wosWOS:000382169300003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCambridge Univ Press
dc.relation.ispartofPlant Genetic Resources-Characterization and Utilization
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectDNA content
dc.subjectgenetic relationship
dc.subjectgenome size
dc.subjectinter-simple sequence repeat
dc.subjectpolymorphism information content
dc.subjectsequence-related amplified polymorphism
dc.subjectVicia sativa
dc.titleSequence-related amplified polymorphism and inter-simple sequence repeat marker-based genetic diversity and nuclear DNA content variation in common vetch (Vicia sativa L.)
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Iskender Tiryaki_Makale.pdf
Boyut:
211.49 KB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tarımsal Biyoteknoloji Bölümü Koleksiyonu