Hyperbaric oxygen mitigates KIM-1 and inflammatory cytokine levels in kidney transplantation

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dc.authoridOztopuz, Ozlem/0000-0002-1373-6311
dc.contributor.authorBuyuk, Basak
dc.contributor.authorOztopuz, Ozlem
dc.date.accessioned2025-01-27T20:22:24Z
dc.date.available2025-01-27T20:22:24Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBackground: The aim of this study was to investigate the effect of hyperbaric oxygen (HBO2) administration during cold ischemic time to organs removed from donors before kidney transplantation. A total of 24 rats were divided into three groups: Group 1 was the control group, Group 2 received 60 minutes of HBO 2 at 2.5 atmospheres absolute, and Group 3 received 120 minutes of 2.5 ATA HBO2. The renal artery was entered with a polyethylene catheter and perfused with a standard organ preservation solution. Falcon tubes containing organs obtained from rats in Groups 2 and 3 were placed in a box supported by ice blocks. The temperature was kept constant at 4 degrees C and the box was placed in a pressure tank with 2.5 ATA HBO2. HBO2 was applied for 60 and 120 minutes, respectively. Organ samples were harvested at the end of 24 hours for histopathological evaluation, immunohistochemical analysis of TNF-alpha and IL- 18, TUNEL analysis for apoptosis, and gene expression levels of kidney injury molecule-1 ( KIM-1) and caspase-3. In histopathological examinations, hematoxylin and eosin staining was performed and samples were evaluated for tubular necrosis and vacuolization criteria. Group 2 and Group 3 had significant decreases compared to Group one in this regard. Immunohistochemical staining was performed for TNF-alpha, IL-18, and apoptosis levels; significant decreases were found in Groups 2 and 3. There were significant decreases in Groups 2 and 3 for KIM-1 and caspase-3 gene expression levels compared to Group 1, as well. Thus, it was demonstrated that during the cold ischemic period before kidney transplantation, HBO2 administration to organs removed from donors can reduce apoptotic cell numbers, inflammatory cytokine release, and histopathological damage to the organs as well as decreasing the expression of the KIM-1 gene, which is an indicator of kidney damage.
dc.identifier.endpage300
dc.identifier.issn1066-2936
dc.identifier.issue3
dc.identifier.pmid37708062
dc.identifier.scopus2-s2.0-85171358529
dc.identifier.scopusqualityQ3
dc.identifier.startpage289
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21868
dc.identifier.volume50
dc.identifier.wosWOS:001162611400006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherUndersea & Hyperbaric Medical Soc Inc
dc.relation.ispartofUndersea and Hyperbaric Medicine
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectcold ischemia
dc.subjecthyperbaric oxygenation
dc.subjectinterleukin-18
dc.subjectkidney
dc.subjectorgan transplantation
dc.subjectKIM-1
dc.subjectrat
dc.titleHyperbaric oxygen mitigates KIM-1 and inflammatory cytokine levels in kidney transplantation
dc.typeArticle

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