An impedimetric biosensor system based on disposable graphite paper electrodes: Detection of ST2 as a potential biomarker for cardiovascular disease in human serum

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dc.authorid000-0002-8186-0461en_US
dc.authorid0000-0003-3042-1087en_US
dc.authorscopusid56106371600en_US
dc.authorscopusid6506764918en_US
dc.authorwosidCMO-5372-2022en_US
dc.authorwosidAAY-1439-2021en_US
dc.contributor.authorDemirbakan, Burcak
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2024-12-19T11:07:07Z
dc.date.available2024-12-19T11:07:07Z
dc.date.issued2021en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.description.abstractIn present study, we developed a highly sensitive, electrochemical immunosensor based on fullerene C60-modified disposable graphite paper (GP) electrode for determination of Suppression of Tumorigenicity 2 (ST2) in human serum. The synthesis of the ST2 immunosensor was monitored with electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) techniques and single frequency impedance (SFI) technique which is utilized for the specific interaction between anti-ST2 and ST2 antigen. Moreover, the morphological alteration of each GP surface was examined by scanning electron microscopy (SEM), SEM-energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM). All parameters such as fullerene C60 concentration, antibody concentration and antibody incubation time were optimized. Analytical characteristics such as linear determination range, repeatability, reproducibility, regeneration and surface coverage were determined for the immunosensor. The ST2 electrochemical immunosensor had excellent repeatability, reproducibility and a wide detection range (from 0.1 fg mL−1 to 100 fg mL−1). The proposed immunosensor also had low limit of detection (LOD) and limit of quantification (LOQ) values of 0.124 fg mL−1 and 0.414 fg mL−1, respectively. The proposed immunosensor was applied to real samples to test applicability in clinical practice.en_US
dc.identifier.citationDemirbakan, B. & Sezgintürk, M. K.. (2021). An impedimetric biosensor system based on disposable graphite paper electrodes: Detection of ST2 as a potential biomarker for cardiovascular disease in human serum. Analytica Chimica Acta, 1144, 43–52. https://doi.org/10.1016/j.aca.2020.12.001 ‌en_US
dc.identifier.doi10.1016/j.aca.2020.12.001
dc.identifier.endpage52en_US
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.pmid33453796
dc.identifier.scopus2-s2.0-85097336136
dc.identifier.startpage43en_US
dc.identifier.urihttps://doi.org/10.1016/j.aca.2020.12.001
dc.identifier.urihttps://hdl.handle.net/20.500.12428/6766
dc.identifier.volume1144en_US
dc.identifier.wosWOS:000612215500005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorDemirbakan, Burcak
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevier B.V.en_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiosensoren_US
dc.subjectCardiovascular diseaseen_US
dc.subjectGraphite paper (GP)en_US
dc.subjectSuppression of tumorigenicity 2 (ST2)en_US
dc.titleAn impedimetric biosensor system based on disposable graphite paper electrodes: Detection of ST2 as a potential biomarker for cardiovascular disease in human serum
dc.typeArticle

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