Development of a biosensor platform based on ITO sheets modified with 3-glycidoxypropyltrimethoxysilane for early detection of TRAP1

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dc.authoridVURAL, Berfin/0000-0003-2752-9708
dc.contributor.authorVural, Berfin
dc.contributor.authorCaliskan, Meltem
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:41:15Z
dc.date.available2025-01-27T20:41:15Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe aim of this research was to design an electrochemical immunosensor for determination of tumour necrosis factor receptor-associated protein-1(TRAP1) antigen, a heat shock protein linked to tumour necrosis factor. The indium-tin oxide covered polyethylene terephthalate (ITO-PET) electrode surface was cleaned and was prepared for the introduction of hydroxyl groups on its surface by using NH4OH/H2O2/H2O. As a silanization agent for covalent attachment of anti-TRAP1 on the surface of the ITO working electrode, 3-glycidoxypropyltrimethoxysilane (3-GOPS) was used. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the immobilization steps. A variety of parameters, 3-GOPS and anti-TRAP1 concentrations, and anti-TRAP1 and TRAP1 incubation durations were optimized. After determining the optimum conditions, characterization studies such as repeatability, reproducibility, regeneration, square wave voltammetry, and single frequency impedance were performed. The electrochemical immunosensor has presented an extremely wide determination range for TRAP1 from 0.1 pg/mL to 100 pg/mL.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [113 Z 678]; Turkish Academy of Sciences (TUBA)
dc.description.sponsorshipWe are grateful for the financial support of this research by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project number: 113 Z 678) and Turkish Academy of Sciences (TUBA).
dc.identifier.doi10.3906/kim-1909-53
dc.identifier.endpage471
dc.identifier.issn1300-0527
dc.identifier.issue2
dc.identifier.pmid33488170
dc.identifier.scopus2-s2.0-85085371190
dc.identifier.scopusqualityQ3
dc.identifier.startpage461
dc.identifier.trdizinid335263
dc.identifier.urihttps://doi.org/10.3906/kim-1909-53
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/335263
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24081
dc.identifier.volume44
dc.identifier.wosWOS:000522796000015
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Chemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subject3-GOPS
dc.subjectITO-PET
dc.subjectTRAP1
dc.subjectimmunosensor
dc.subjectbiosensor
dc.titleDevelopment of a biosensor platform based on ITO sheets modified with 3-glycidoxypropyltrimethoxysilane for early detection of TRAP1
dc.typeArticle

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