Assessment of Protein Quantity and Quality of Turkish Maize Landraces with Different Opacity Levels

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dc.authoridBaytekin Karaoğlu, Gülhan / 0000-0002-7094-8978
dc.authoridSongur, Umut / 0000-0001-7035-9607
dc.authoridKahrıman, Fatih / 0000-0001-6944-0512
dc.contributor.authorBaytekin Karaoğlu, Gülhan
dc.contributor.authorSongur, Umut
dc.contributor.authorÇiğdem, Esra
dc.contributor.authorŞimşek, Gökalp
dc.contributor.authorKahrıman, Fatih
dc.date.accessioned2025-01-27T18:56:24Z
dc.date.available2025-01-27T18:56:24Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractProtein ratio and amino acid composition vary considerably in maize according to the level of opacity. In this study, the total protein content, amounts of some essential amino acids (lysine and tryptophan) and quantitative and qualitative variation of protein fractions in maize seed samples separated according to different opacity levels were investigated. In the study, 6 local maize landraces with opaque kernel structure and 3 standards were used. The field trial was carried out in 2021 according to the complete blocks trial design with 3 replications. Samples of local maize landraces from the field experiment were separated on a light table according to 5 different opacity levels (0%, 25%, 50%, 75% and 100%). Protein content, lysine and tryptophan contents, albumin + globulin, glutelin and zein contents were analyzed in the samples separated by their opacity. In addition, protein fractions were subjected to SDS-PAGE analysis and the changes in protein bands according to opacity level were examined. Differences between genotypes, opacity levels and their interaction were illustrated with box plot and PCA-Biplots. Two-way dendrograms were created for qualitative discrimination. The results of the study showed that there were significant changes in protein amount and quality according to the opacity levels of the genotypes. The protein contents of the genotypes were 5.95–10.22%, lysine contents 0.33–0.46%, tryptophan contents 0.032–0.083%, albumin + glubulin contents 1.92–2.72%, glutelin contents 0.64–1.08%, and zein contents 1.59–1.99%. There was a significant difference in presence or absence according to the opacity levels in the protein fractions.
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (122O733)
dc.identifier.doi10.15414/afz.2024.27.01.58-67
dc.identifier.endpage67
dc.identifier.issn1335-258X
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85191988388
dc.identifier.scopusqualityQ4
dc.identifier.startpage58
dc.identifier.urihttps://doi.org/10.15414/afz.2024.27.01.58-67
dc.identifier.urihttps://hdl.handle.net/20.500.12428/12971
dc.identifier.volume27
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSlovak University of Agriculture in Nitra
dc.relation.ispartofActa Fytotechnica et Zootechnica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250125
dc.subjectessential amino acid
dc.subjectfood
dc.subjectgenetic resources
dc.subjectZea mays
dc.titleAssessment of Protein Quantity and Quality of Turkish Maize Landraces with Different Opacity Levels
dc.typeArticle

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