Evaluation of Smooth Muscle Myosin Heavy Chain Isoform Expressions in a Buried Penis

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dc.authoridOztopuz, Ozlem/0000-0002-1373-6311
dc.contributor.authorKurtulus, Senay
dc.contributor.authorSuzen, Alev
dc.contributor.authorSılan, Fatma
dc.contributor.authorOztopuz, Rahime Ozlem
dc.date.accessioned2025-01-27T21:04:10Z
dc.date.available2025-01-27T21:04:10Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBackground: A buried penis (BP) is rare in which the penile body is retracted into the prepubic adipose tissue. This research focuses on differences in smooth muscle myosin heavy chain (SMMHC) isoform expressions in the dartos fascia. Methods: A total of 82 children, 41 of whom had BPs, who applied for circumcision between May and November 2021, were included in the study. The cases were divided into four groups aged >= 6 years (NP6, n = 18) and aged <= 3 years (NP3, n = 17) with normal penile appearance, aged >= 6 years (BP6, n = 23) and aged <= 3 years (BP,n = 24) with a BP. SMMHC isoforms mRNA gene expression analyses were performed by quantitative PCR technique in dartos fascia obtained from foreskin removed by circumcision. Results: Compared to the NP3 group, the SM1 mRNA expressed in the BP6 group was statistically significantly higher (p < 0.005). SM2 mRNA levels expressed in dartos fascia were considerably higher in NP6 and NP3 groups compared to BP6 and BP3 groups (p < 0.001). The SM2/SM1 ratio was 0.85 in the BP6 group and 1.46 in the NP6 group, which was statistically significant (p = 0.006) and increased from 0.87 in the BP3 group to 2.21 in the NP3 group (p < 0.001). Conclusion: In a buried penis, there is a difference in the expression of SMMHC isoforms. SM1 is highly expressed, while SM2 decreases, increasing the SM2/SM1 ratio. This causes increased contractility in the smooth muscle, leading to retraction of the penile body. The dartos fascia surrounding it resembles aberrant muscle tissue in boys with a BP. Level of Evidence: Level III. Type of Study: Case-control study. (c) 2024 Elsevier Inc. All rights reserved.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit [3764, TSA-2022-3764]
dc.description.sponsorshipThe research titled EVALUATION OF SMOOTH MUSCLE MYOSIN HEAVY CHAIN ISOFORM EXPRESSIONS IN A BURIED PENIS (Project ID:3764, Project Code: TSA-2022-3764) was supported by Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit.
dc.identifier.doi10.1016/j.jpedsurg.2024.03.036
dc.identifier.endpage1530
dc.identifier.issn0022-3468
dc.identifier.issn1531-5037
dc.identifier.issue8
dc.identifier.pmid38631998
dc.identifier.scopus2-s2.0-85190511286
dc.identifier.scopusqualityQ1
dc.identifier.startpage1526
dc.identifier.urihttps://doi.org/10.1016/j.jpedsurg.2024.03.036
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27572
dc.identifier.volume59
dc.identifier.wosWOS:001273792500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherW B Saunders Co-Elsevier Inc
dc.relation.ispartofJournal of Pediatric Surgery
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBuried penis
dc.subjectDartos facia
dc.subjectSM1
dc.subjectSM2
dc.subjectSmooth muscle myosin heavy chain
dc.titleEvaluation of Smooth Muscle Myosin Heavy Chain Isoform Expressions in a Buried Penis
dc.typeArticle

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