Chitinolytic Bacillus subtilis Ege-B-1.19 as a biocontrol agent against mycotoxigenic and phytopathogenic fungi

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dc.authoridOztopuz, Ozlem/0000-0002-1373-6311
dc.authoridLiaqat, Fakhra/0000-0001-7007-8936
dc.authoridELTEM, RENGIN/0000-0002-0642-7676
dc.contributor.authorOztopuz, Ozlem
dc.contributor.authorSarigul, Nermin
dc.contributor.authorLiaqat, Fakhra
dc.contributor.authorPark, Ro-Dong
dc.contributor.authorEltem, Rengin
dc.date.accessioned2025-01-27T20:16:32Z
dc.date.available2025-01-27T20:16:32Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBackground: Biological control of pathogenic fungi is a possible alternate to the chemical control, which is harmful to humans and environment. Soil-borne Bacillus strains can be potential biocontrol agents and a source of lytic enzymes. Aim: This study aimed to examine biocontrol potential and lytic enzyme activities of a soil isolate Bacillus subtilis Ege-B-1.19. Materials and methods: Strain was identified by biochemical and 16S rRNA gene analysis and its biocontrol activity was investigated against Aspergillus niger EGE-K-213, Aspergillus foetidus EGE-K-211, Aspergillus ochraceus EGE-K-217, Fusarium solani KCTC6328, Rhizoctonia solani KACC40111 and Colletotrichum gloeosporioides KACC40689. Chitinase, chitosanase, N-acetyl-beta-hexosaminidase and protease activities of B. subtilis Ege-B-1.19 were also determined. Chitosanase was purified using Sephadex G-150 column and its molecular weight was determined by SDS-PAGE. Chitooligosaccharides production using chitosanase was carried out and analysed by TLC and HPLC. Results: Results depicted that B. subtilis Ege-B-1.19 has shown inhibitory effects against all the test fungi. Chitinase, chitosanase, N-acetyl-beta-hexosaminidase and protease activities were determined as 2.7 U mL(-1), 7.2 U 6.2 U mL(-1) and 12.2 U mL(-1), respectively. Molecular weight of purified chitosanase was 44 kDa. Chitosanase hydrolysed chitosan to glucosamine (GlcN), dimers (GlcN)(2) and trimers (GlcN)(3). Conclusion: Bacillus subtilis Ege-B-1.19 can be anticipated as useful biocontrol agent and its chitosanase can be utilized for enzymatic synthesis of chitooligosaccharides.
dc.description.sponsorshipResearch Project fund of TUBITAK [104O339]
dc.description.sponsorshipThis work was supported by the Research Project fund of TUBITAK (104O339).
dc.identifier.doi10.1515/tjb-2018-0144
dc.identifier.endpage331
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85073684114
dc.identifier.scopusqualityQ3
dc.identifier.startpage323
dc.identifier.trdizinid335430
dc.identifier.urihttps://doi.org/10.1515/tjb-2018-0144
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/335430
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21272
dc.identifier.volume44
dc.identifier.wosWOS:000481592100011
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisi
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectVineyard soil
dc.subjectBacillus subtilis
dc.subjectChitosanase
dc.subjectBiocontrol
dc.subjectChitooligosaccharides
dc.titleChitinolytic Bacillus subtilis Ege-B-1.19 as a biocontrol agent against mycotoxigenic and phytopathogenic fungi
dc.typeArticle

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