Synthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase

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dc.authoridŞahiner, Mehtap / 0000-0001-8666-7954
dc.authoridŞahiner, Nurettin / 0000-0003-0120-530X
dc.contributor.authorAltunbaş, Canan
dc.contributor.authorAslan, Ahmet
dc.contributor.authorKusat, Kevser
dc.contributor.authorŞahiner, Mehtap
dc.contributor.authorAkgöl, Sinan
dc.contributor.authorŞahiner, Nurettin
dc.date.accessioned2025-01-27T20:11:59Z
dc.date.available2025-01-27T20:11:59Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.descriptionThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
dc.description.abstractThe advantages of cryogels for enzyme immobilization applications include their mechanical and chemical robustness, ease of production, superior porosity, and low cost. Currently, many researchers are exploring porous material-based systems for enzyme immobilization that are more efficient and economically viable. Here, poly(2-Hydroxyethyl methacrylate-co-allyl glycidyl ether) (p(HEMA-co-AGE)) cryogel matrices were synthesized via the free radical cryopolymerization method to be employed as the support material. For the immobilization of the catalase enzyme onto the p(HEMA-co-AGE) cryogel matrix (catalase@p(HEMA-co-AGE), the best possible reaction conditions were determined by altering parameters such as pH, catalase initial concentration, and flow rate. The maximum catalase immobilization amount onto the p(HEMA-co-AGE) cryogel was found to be 48 mg/g cryogel. To determine the advantages of the cryogel matrix, e.g., the stability and reusability of the cryogel matrix, the adsorption-desorption cycles for the catalase enzyme were repeated five times using the same cryogel matrix. At the end of the reusability tests, it was found that the cryogel was very stable and maintained its adsorption capacity with the recovery ratio of 93.8 +/- 1.2%. Therefore, the p(HEMA-co-AGE) cryogel matrix affords repeated useability, e.g., up to five times, without decreasing its catalase binding capacities significantly and has promising potential for many industrial applications. Cryogels offer clear distinctive advantages over common materials, e.g., micro/nano particles, hydrogels, films, and composites for these applications. At present, many researchers are working on the design of more effective and economically feasible, porous material-based systems for enzyme immobilization
dc.identifier.doi10.3390/gels8080501
dc.identifier.issn2310-2861
dc.identifier.issue8
dc.identifier.pmid36005102
dc.identifier.scopus2-s2.0-85137393333
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.3390/gels8080501
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20802
dc.identifier.volume8
dc.identifier.wosWOS:000845890100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMDPI
dc.relation.ispartofGels
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectenzyme immobilization
dc.subjectcryogel
dc.subjectcatalase
dc.subjectsuperporous polymer network
dc.titleSynthesis and Characterization of a New Cryogel Matrix for Covalent Immobilization of Catalase
dc.typeArticle

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