Assessment of the genotoxic potential of tetrachlorvinphos insecticide by cytokinesis-block micronucleus and sister chromatid exchange assays

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dc.authorid0000-0001-9640-3354en_US
dc.authorid0000-0002-2014-6635en_US
dc.authorscopusid57195554629en_US
dc.authorscopusid22733342800en_US
dc.authorwosidCKQ-7747-2022en_US
dc.authorwosidDVQ-2433-2022en_US
dc.contributor.authorÇobanoğlu, Hayal
dc.contributor.authorÇayır, Akın
dc.date.accessioned2022-12-31T11:36:33Z
dc.date.available2022-12-31T11:36:33Z
dc.date.issued2021en_US
dc.departmentMeslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü
dc.description.abstractTetrachlorvinphos is an organophosphate that is classified as a carcinogen in humans by several authorities. Due to very limited data regarding the genotoxic potential, we aimed to comprehensively investigate in vitro genotoxic potential of tetrachlorvinphos. We performed our study by applying the cytokinesis-block micronucleus cytome and sister chromatid exchange (SCE) assays to human peripheral blood lymphocytes. We evaluated micronucleus (MN) and SCE frequencies and cytokinesis-block proliferation index in both exposed and non-exposed lymphocytes. We also calculated the chromosomal instability level in response to exposure by combining the results of MN and SCE. We found that MN frequency did not increase with exposure to tetrachlorvinphos (0–50 µg/ml). In contrast, we observed that SCE frequencies significantly increased with exposure to ≥5 µg/ml tetrachlorvinphos. Furthermore, exposure to tetrachlorvinphos at concentrations of 50 µg/ml induced a significant increase in chromosomal instability level (p < 0.05). Cytokinesis-block proliferation index level did not significantly decrease in response to tetrachlorvinphos exposure. Our findings reveal that tetrachlorvinphos resulted in different DNA damages that were measured by two assays. Furthermore, our findings suggested that exposure to tetrachlorvinphos increased chromosomal instability that is a hallmark of many malignancies. We conclude that although tetrachlorvinphos does not significantly increase the MN level, the significant increase of both SCE and CIN frequencies indicates the genotoxic potential of tetrachlorvinphos in human peripheral lymphocytes. Additionally, tetrachlorvinphos is not cytotoxic in the range of tested concentrations.en_US
dc.identifier.citationCobanoglu, H., & Cayir, A. (2021). Assessment of the genotoxic potential of tetrachlorvinphos insecticide by cytokinesis-block micronucleus and sister chromatid exchange assays. Human and Experimental Toxicology, 40(12_suppl), S158-S163. doi:10.1177/09603271211036126en_US
dc.identifier.doi10.1177/09603271211036126
dc.identifier.endpageS163en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue12/Supplement 12en_US
dc.identifier.pmid34334017
dc.identifier.scopus2-s2.0-85112039690
dc.identifier.startpageS158en_US
dc.identifier.urihttps://doi.org/10.1177/09603271211036126
dc.identifier.urihttps://hdl.handle.net/20.500.12428/3775
dc.identifier.volume40en_US
dc.identifier.wosWOS:000680620700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÇobanoğlu, Hayal
dc.institutionauthorÇayır, Akın
dc.language.isoen
dc.publisherSAGE Publicationsen_US
dc.relation.ispartofHuman and Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTetrachlorvinphosen_US
dc.subjectGenotoxicityen_US
dc.subjectSister Chromatid Exchangeen_US
dc.subjectMicronucleusen_US
dc.subjectGenomic Instabilityen_US
dc.titleAssessment of the genotoxic potential of tetrachlorvinphos insecticide by cytokinesis-block micronucleus and sister chromatid exchange assays
dc.typeArticle

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