EVALUATION OF QUORUM SENSING SIGNALS OF STRONG BIOFILM PRODUCING BACTERIA VIA LC-MSMS, HPLC AND BIOSENSORS

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dc.contributor.authorIpek, Dilvin
dc.date.accessioned2025-01-27T20:38:36Z
dc.date.available2025-01-27T20:38:36Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThis study aimed to show presence of Quorum Sensing (QS) signals of Gramnegative and Gram-positive biofilm producing bacteria isolated from real dairy process lines. Defining the profile and chemical composition of QS-signals is an important factor in control of microbial resistance and biofilm production. We especially focused on unusual behaviour of Gram-positive and Gram-negative isolates. Long-chain acyl-homoserine lactones (AHLs) signals (C14-HSL, C16-HSL and C18-HSL) and DFD (4,5-dihidroksi-2,3-pentanedione)-AI2 signals of the isolates were studied by High-performance liquid chromatography (HPLC) and Liquid Chromatography with tandem mass spectrometry (LC-MSMS) methods. All Gram-positive isolates were defined as AHL-producers. All Gram-negative isolates, formerly defined as non-AHL producers by both biosensors and HPLC methods, were identified as AHL-producers. DFD signal was only detected from Gram-negative Klebsiella pneumonia, Enterobacter cloacae and Klebsiella oxytoca isolates. The results demonstrated that the QS system is a complex system and biosensor microorganism may not be the best method for QS-signal identification. The results also provided new insights in defining the profile and chemical composition of QS-signals importance for interrupting the chemical communication completely to reduce biofilm formation and prevent resistance gain of microorganisms.
dc.description.sponsorshipScientific Research Project Commission of Canakkale Onsekiz Mart University [FBA-2018-1438]
dc.description.sponsorshipThis research was supported by the Scientific Research Project Commission of Canakkale Onsekiz Mart University (FBA-2018-1438) .
dc.identifier.doi10.23902/trkjnat.1276562
dc.identifier.endpage30
dc.identifier.issn2528-9691
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85205589894
dc.identifier.scopusqualityN/A
dc.identifier.startpage23
dc.identifier.trdizinid1201954
dc.identifier.urihttps://doi.org/10.23902/trkjnat.1276562
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1201954
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23660
dc.identifier.volume24
dc.identifier.wosWOS:001088177900003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTrakya Univ Balkan Yerlesesi Enstituler Binasi
dc.relation.ispartofTrakya University Journal of Natural Sciences
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectAHL Signals
dc.subjectAI-2 Signals
dc.subjectBiofilm
dc.subjectBiosensors
dc.subjectHPLC
dc.subjectLC-MSMS
dc.titleEVALUATION OF QUORUM SENSING SIGNALS OF STRONG BIOFILM PRODUCING BACTERIA VIA LC-MSMS, HPLC AND BIOSENSORS
dc.typeArticle

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