Immobilisation of Aspergillus oryzae ?-amylase and Aspergillus niger glucoamylase enzymes as cross-linked enzyme aggregates

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dc.authoridSAHUTOGLU, Arif Sercan/0000-0003-1183-3553
dc.contributor.authorSahutoglu, A. Sercan
dc.contributor.authorAkgul, Cahit
dc.date.accessioned2025-01-27T20:22:27Z
dc.date.available2025-01-27T20:22:27Z
dc.date.issued2015
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractCross-linked enzyme aggregates (CLEA) of Aspergillus oryzea a-amylase (AoAA) and Aspergillus niger glucoamylase (AnGA) were prepared using glutaraldehyde and dextran polyaldehyde as crosslinkers. The maximum activity recoveries for glutaraldehyde cross-linking were 21.8 % and 41.2 %, respectively. The addition of a proteic feeder (bovine serum albumin) exhibited a negative effect on the activity recoveries for both enzymes. Dextran polyaldehyde was used as a cross-linking agent instead of glutaraldehyde to reduce the activity losses. As a result, an activity recovery of 60.0 % was obtained for Aspergillus oryzea a-amylase. On the other hand, no activity recovery was observed for Aspergillus niger glucoamylase due to the latter enzymes affinity for dextran.(C) 2014 Institute of Chemistry, Slovak Academy of Sciences
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit [2012/039]
dc.description.sponsorshipThis research was supported by the Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit (2012/039).
dc.identifier.doi10.1515/chempap-2015-0031
dc.identifier.endpage439
dc.identifier.issn0366-6352
dc.identifier.issn1336-9075
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84922215083
dc.identifier.scopusqualityQ2
dc.identifier.startpage433
dc.identifier.urihttps://doi.org/10.1515/chempap-2015-0031
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21894
dc.identifier.volume69
dc.identifier.wosWOS:000350134900007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherDe Gruyter Open Ltd
dc.relation.ispartofChemical Papers
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectimmobilisation
dc.subjectglucoamylase
dc.subjectalpha-amylase
dc.subjectglutaraldehyde
dc.subjectdextran polyaldehyde
dc.subjectcrosslinked enzyme aggregates (CLEAs)
dc.titleImmobilisation of Aspergillus oryzae ?-amylase and Aspergillus niger glucoamylase enzymes as cross-linked enzyme aggregates
dc.typeArticle

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