Immobilisation of Aspergillus oryzae ?-amylase and Aspergillus niger glucoamylase enzymes as cross-linked enzyme aggregates
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Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
De Gruyter Open Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cross-linked enzyme aggregates (CLEA) of Aspergillus oryzea a-amylase (AoAA) and Aspergillus niger glucoamylase (AnGA) were prepared using glutaraldehyde and dextran polyaldehyde as crosslinkers. The maximum activity recoveries for glutaraldehyde cross-linking were 21.8 % and 41.2 %, respectively. The addition of a proteic feeder (bovine serum albumin) exhibited a negative effect on the activity recoveries for both enzymes. Dextran polyaldehyde was used as a cross-linking agent instead of glutaraldehyde to reduce the activity losses. As a result, an activity recovery of 60.0 % was obtained for Aspergillus oryzea a-amylase. On the other hand, no activity recovery was observed for Aspergillus niger glucoamylase due to the latter enzymes affinity for dextran.(C) 2014 Institute of Chemistry, Slovak Academy of Sciences
Açıklama
Anahtar Kelimeler
immobilisation, glucoamylase, alpha-amylase, glutaraldehyde, dextran polyaldehyde, crosslinked enzyme aggregates (CLEAs)
Kaynak
Chemical Papers
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
69
Sayı
3
