Determining Total Protein and Bioactive Protein Concentrations in Bovine Colostrum
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Journal of Visualized Experiments
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Colostrum is a complex biological fluid produced by mammals immediately after parturition. It meets all the nutritional requirements for neonates as a good source of macro-and micronutrients, bioactive peptides, and growth factors. Bovine colostrum is also a potential source of nutrition and bioactive because of its rich protein content that includes immunoglobulin G (IgG) and lactoferrin. However, the level of lactoferrin and IgG in bovine colostrum changes markedly during the lactation period. Therefore, monitoring the concentration of IgG and lactoferrin for the use of bovine colostrum as a protein source is an important question to study. Methods in this article describe how to determine protein content, as well as specific concentrations of lactoferrin and IgG. These methods include the following steps: Isolation of bovine colostrum proteins, Determination of protein concentration via Bicinchoninic acid assay (BCA), Visualization of proteins via SDS-PAGE, Determination of lactoferrin, and IgG concentration using an ELISA Assay.
Açıklama
Anahtar Kelimeler
Animals, Cattle, Colostrum, Enzyme-Linked Immunosorbent Assay, Female, Immunoglobulin G, Lactation, Mammals, Pregnancy
Kaynak
Journal of Visualized Experiments
WoS Q Değeri
Q3
Scopus Q Değeri
Cilt
Sayı
178
Künye
Arslan, A., Duman, H., Kaplan, M., Uzkuç, H., Bayraktar, A., Ertürk, M., . . . Karav, S. (2021). Determining total protein and bioactive protein concentrations in bovine colostrum. Journal of Visualized Experiments, 2021(178) doi:10.3791/63001
