A novel and disposable GP- based impedimetric biosensor using electropolymerization process with PGA for highly sensitive determination of leptin: Early diagnosis of childhood obesity

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dc.authorid0000-0002-5123-5972en_US
dc.authorid0000-0003-3042-1087en_US
dc.authorscopusid56106034900en_US
dc.authorscopusid6506764918en_US
dc.authorwosidAAY-1451-2021en_US
dc.authorwosidAAY-1439-2021en_US
dc.contributor.authorÖzcan, Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2023-04-10T12:03:24Z
dc.date.available2023-04-10T12:03:24Z
dc.date.issued2021en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.description.abstractThis study presents a novel, single-use electrochemical biosensor for the leptin biomarker, which may have potential use for early diagnosis of childhood obesity. The graphite paper working electrode was used for the first time in impedimetric biosensors. All immobilization procedure, investigation of the optimal parameters and characterization of biosensors were followed and evaluated using Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV). The Scanning Electron Microscope (SEM) was utilized to visualize the morphology of the electrode surface during the immobilization steps of the immunosensor. Moreover, the characterization of the interactions between anti-leptin and leptin was investigated by using Single Frequency Technique (SFI). The applicability of the designed biosensor for real serum samples was tested for clinical use. It was observed that the biosensor allows high sensitivity in the analyte detection (leptin) in real serum samples. Moreover, it was suggested that the developed biosensor presents advantages such as long shelf life (5% loss of activity after 8 weeks and 60% loss after 10 weeks), ability to determine analyte concentrations at picogram level (0.2 pg mL−1 -20 pg mL−1), low limit of detection (0.00813 pg mL− 1), reproducibility, reusability (12 times) and high sensitivity.en_US
dc.identifier.citationÖzcan, B., & Sezgintürk, M. K. (2021). A novel and disposable GP- based impedimetric biosensor using electropolymerization process with PGA for highly sensitive determination of leptin: Early diagnosis of childhood obesity. Talanta, 225 doi:10.1016/j.talanta.2020.121985en_US
dc.identifier.doi10.1016/j.talanta.2020.121985
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid33592733
dc.identifier.scopus2-s2.0-85097651723
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2020.121985
dc.identifier.urihttps://hdl.handle.net/20.500.12428/3988
dc.identifier.volume225en_US
dc.identifier.wosWOS:000652032600052
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzcan, Burcu
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/113 Z 678
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLeptinen_US
dc.subjectChildhood obesityen_US
dc.subjectBiosensoren_US
dc.subjectGraphite paper electrodeen_US
dc.titleA novel and disposable GP- based impedimetric biosensor using electropolymerization process with PGA for highly sensitive determination of leptin: Early diagnosis of childhood obesity
dc.typeArticle

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