Photoelectrocatalytic determination of NADH in a flow injection system with electropolymerized methylene blue
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Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pergamon-Elsevier Science Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
It was firstly described that a glassy carbon electrode electropolymerized with methylene blue shows an efficient photoelectrocatalytic activity towards NADH oxidation in a phosphate buffer solution (pH 7.0). In order to perform the photoelectrocatalytic determination of NADH in a flow injection analysis (FIA) system, a home-made flow electrochemical cell with a suitable transparent window for the irradiation of the electrode surface was constructed. The currents obtained from the photoamperometric measurements in the FIA system at optimum conditions (flow rate of carrier solution, 1.3 mL min(-1): transmission tubing length, 10 cm; injection volume, 100 mu L; and constant applied potential, +150 mV vs. Ag/AgCl) were linearly dependent on the NADH concentration and linear calibration curves were obtained in the range of 1.0 x 10(-7)-2.0 x 10(-4) M. The detection limit was found to be 4.0 x 10(-8) M for photoamperometric determination of NADH. (C) 2010 Elsevier Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
Photoelectrocatalysis, Flow injection analysis, Modified electrode, NADH, Methylene blue
Kaynak
Electrochimica Acta
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
56
Sayı
3
