Demonstration of linkage and development of the first low-density genetic map of garlic, based on AFLP markers

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dc.authoridİpek, Meryem / 0000-0002-0609-3442
dc.contributor.authorİpek, Meryem
dc.contributor.authorİpek, A.
dc.contributor.authorAlmquist, SG
dc.contributor.authorSimon, PW
dc.date.accessioned2025-01-27T20:47:46Z
dc.date.available2025-01-27T20:47:46Z
dc.date.issued2005
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractGarlic (Allium sativum L.) is a long-cultivated, clonally propagated diploid plant (2n=2x=16). With routine seed production now underway, we used populations (MP1 and MP2) generated by self-pollination of unrelated plants to generate two low-density genetic maps of garlic, consisting of amplified fragment length polymorphism (AFLP) and gene-specific markers. We did not observe any two plants with identical marker patterns in either population, indicating that they were the result of amphimixis rather than apomixis. This is an important finding, since several Alliums are facultative apomicts. A total of 360 markers segregated in MP1 (12.8 AFLP markers per primer combination) and 321 markers segregated in MP2 (13.9 per primer combination) to indicate a fairly high level of genetic heterozygosity in the garlic nuclear genome. Of these markers, 15.3% in MP1 and 24.3% in MP2 had segregation ratios distorted from the expected 3: 1. Interestingly, 94.7% of those distorted segregations fit a 15: 1 segregation ratio for duplicated loci, suggesting extensive levels of duplication in the garlic genome and supporting similar observations for onion. The genetic map for the MP1 family with 216 markers spanned 1,166 cM of the garlic genome ( 5.4 cM average), while 143 markers of MP2 spanned 862 cM ( 6.0 cM average). Gene-specific markers for alliinase, chitinase, sucrose 1-fructosyltransferase (SST-1), and chalcone synthase (CHS) were mapped, demonstrating the immediate utility of the garlic genetic map. These two garlic families had relatively few segregating AFLP markers in common, which supports their relatively distant relationship based on diversity analysis. Of those markers that were conserved, linkages were also conserved.
dc.identifier.doi10.1007/s00122-004-1815-5
dc.identifier.endpage236
dc.identifier.issn0040-5752
dc.identifier.issn1432-2242
dc.identifier.issue2
dc.identifier.pmid15565379
dc.identifier.scopus2-s2.0-13244262973
dc.identifier.scopusqualityQ1
dc.identifier.startpage228
dc.identifier.urihttps://doi.org/10.1007/s00122-004-1815-5
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25039
dc.identifier.volume110
dc.identifier.wosWOS:000226553900004
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofTheoretical and Applied Genetics
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectNuclear-Dna
dc.subjectAllium
dc.titleDemonstration of linkage and development of the first low-density genetic map of garlic, based on AFLP markers
dc.typeArticle

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