Celiac disease biomarker quantification in human fluid microenvironment: A selective and ultrasensitive magnetosensing immunoplatform

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dc.authoridSezgintürk, Mustafa Kemal / 0000-0003-3042-1087
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-05-29T02:57:57Z
dc.date.available2025-05-29T02:57:57Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractAn innovative magnetosensing strategy for highly sensitive impedimetric determination of anti-tissue transglutaminase antibodies (anti-tTG) was developed and applied successfully in real serum and saliva samples. The transducer surface of the proposed immunosensor consisted of an indium tin electrode (ITO) attached with poly (3-thienylacetic acid) (PTAc)-coated magnetic nanoparticles, which provided a very useful surface for the attachment of the biological molecules. The PTAc-coated magnetic nanoparticles were held by a magnetic field on the electrode surface, and the immunological reaction was carried out on magnetic nanoparticles as a solid platform on which the tissue transglutaminase (tTG) was covalently bound. With the specific capture of anti-tTG on the tTG-immobilized surface, an impedimetric signal was measured, and the electrochemical response of this specific reaction was correlated with the anti-tTG concentration. A linear relationship between the impedimetric signal and the anti-tTG concentration was obtained over a wide range of 0.125-25 U/mL. This magnetobiosensor illustrated a stable quantitative signal to anti-tTG concentrations after 45 minutes of incubation with a limit of detection of 0.034 U/mL and a low relative standard deviation (RSD) of 3.61 %, n = 3. This immunosensor's electrochemical behaviour was thoroughly examined, with consideration given to factors including sensitivity, specificity, repeatability, reproducibility, and storage stability. Lastly, serum and saliva samples were analyzed using the biosensor, and excellent correlation was achieved between the commercial ELISA kit and the proposed immunosensor. As a result, this approach held out a lot of hope for a straightforward, affordable, and user-friendly analytical technique that would enable the label-free measurement of anti-tTG levels.
dc.description.sponsorshipScientific and Technological Research Council of Tekirdag Namimath;k Kemal University within the BAP project [NKUBAP.00.GA.23.465]
dc.description.sponsorshipThe authors gratefully acknowledge funding from the Scientific and Technological Research Council of Tekirdag Nam & imath;k Kemal University within the BAP project (project number: NKUBAP.00.GA.23.465) .
dc.identifier.doi10.1016/j.snb.2025.137504
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85219026395
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://doi.org/10.1016/j.snb.2025.137504
dc.identifier.urihttps://hdl.handle.net/20.500.12428/30204
dc.identifier.volume433
dc.identifier.wosWOS:001439633100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors and Actuators B-Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250529
dc.subjectCeliac disease
dc.subjectMagneto-biosensor
dc.subjectAnti-tissue transglutaminase
dc.subjectElectrochemical immunosensor
dc.titleCeliac disease biomarker quantification in human fluid microenvironment: A selective and ultrasensitive magnetosensing immunoplatform
dc.typeArticle

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