THE SAGA COMPLEX IS ESSENTIAL FOR THE REGULATION OF GENES INVOLVED IN YEAST TREHALOSE METABOLISM

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dc.authoridTURGUT GENC, Tulay/0000-0001-5074-3572
dc.contributor.authorGenc, Tulay Turgut
dc.date.accessioned2025-01-27T20:55:43Z
dc.date.available2025-01-27T20:55:43Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractSaccharomyces cerevisiae accumulates trehalose as a stress metabolite in adverse environmental conditions. The trehalose synthesis and breakdown are important for the regulation of trehalose levels within the yeast cell. Therefore, TPS1 and NTH1 gene expressions are tightly regulated during transcription and also translation. Since both genes contain Stress Response Elements (STRE) in the promoter regions, they are co-activated under stress conditions. However, the presence of similar regulatory elements in the promoter of both genes shows that these genes undergo a different regulation at the transcriptional level. In our study, the role of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex in the transcriptional regulation of TPS1 and NTH1 genes was determined in nutrient-poor environment. For that purpose, the wild type and Delta ada] mutant yeast cells, where Ada1p is a member of the SAGA complex, were grown in normal and nitrogen starvation conditions. In addition, trehalose level was detected enzymatically in both wild type and mutant yeast cells. In silico promoter analysis of TPS1 and NTH1 promoters revealed that the STRE sequences required for binding of Msn2/4 transcription factors are closed by nucleosomes at the NTH1 promoter, but open at the TPS1 promoter. In the absence of Ada1p, stress-induced promoter activation in the TPS1 gene was observed, while NTH1 gene expression was not activated. According to these results, the nucleosomes spanning the STRE sequences could not be mobilized in the absence of Ada1 protein, and therefore the Msn2/4 transcription factors cannot bind to the promoter and activate the NTH1 gene expression under stress conditions. It was also observed that in the absence of Ada1p, trehalose accumulation was reduced regardless of stress conditions.
dc.identifier.doi10.23902/trkjnat.1131400
dc.identifier.endpage176
dc.identifier.issn2528-9691
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85205590659
dc.identifier.scopusqualityN/A
dc.identifier.startpage167
dc.identifier.trdizinid1134513
dc.identifier.urihttps://doi.org/10.23902/trkjnat.1131400
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1134513
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26146
dc.identifier.volume23
dc.identifier.wosWOS:000878534200006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTrakya Univ Balkan Yerlesesi Enstituler Binasi
dc.relation.ispartofTrakya University Journal of Natural Sciences
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectMsn2
dc.subject4
dc.subjectNitrogen starvation
dc.subjectNucleosome
dc.subjectSAGA
dc.subjectTrehalose
dc.titleTHE SAGA COMPLEX IS ESSENTIAL FOR THE REGULATION OF GENES INVOLVED IN YEAST TREHALOSE METABOLISM
dc.typeArticle

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