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Öğe Akut miyokard enfarktüs geçiren kişilerde apolipoprotein E polimorfizmi ile chlamydia pneumoniae taşıyıcılığı arasındaki korelasyonun belirlenmesi(Çanakkale Onsekiz Mart Üniversitesi, 2001) Ay, Mustafa; Erdem, Günhanoz Akut Miyokard Enfarktüs Geçiren Kişilerde Apolipoprotein E Polimorfizmi ile Chlamydia pneumoniae Taşıyıcılığı Arasındaki Korelasyonun Belirlenmesi. Chlamydia pneumoniae'^ karşı gelişen antikorlar ile akut miyokard enfarktüs ve ateroskleroz arasında ilişki olduğu seroepidemiyolojik çalışmalarla saptanmıştır. Son yıllarda, koroner arterlerde oluşan aterom plaklarda, Chlamydia pneumoniae 'nın varlığı gösterilmiştir. Bu tez çalışmasında, akut miyokard enfarktüs geçiren kişilerde, Chlamydia pneumoniae taşıyıcılığı ile APOE polimorfizmi arasındaki olası bir korelasyon araştırılmıştır. Hasta ve kontrol grubu bireylerinden alınan serum örneklerinde, Chlamydia pneumoniae IgG antikorları, ELISA yöntemiyle belirlenmiştir. APOE polimorfizmleri, RFLP tekniği kullanılarak saptanmıştır. Ayrıca, çalışma gruplarını oluşturan bireylere bir anket uygulanarak, kişilere ait yaşam tarzı ve belirli alışkanlıklarla, Chlamydia pneumoniae taşıyıcılığı arasında bir bağlantı olup olmadığı da incelenmiştir. Anahtar kelimeler: Akut miyokard enfarktüs, kardiyovasküler hastalıklar, Chlamydia pneumoniae, apolipoprotein E.Öğe Akut miyokard enfarktüs ve Chlamydia pneumoniae(2002) Ay, Mustafa; Erdem, GünhanAteroskleroz ve akut miyokard enfarktüs ile Chlamydia pneumoniae türüne karşı gelişen antikorlar aracında ilişki olduğu seroepidemiyolojik çalışmalarla saptanmıştır. Son yıllarda koroner arterlerde oluşan aterom plaklarda Chlamydia pneumoniae varlığı gösterilmiştir. Bu çalışmada, 60 miyokard enfarktüslü hasta ile 30 kontrol bireyinde Chlamydia pneumoniae taşıyıcılığı araştırılmıştır. Hasta ve kontrol grubunu oluşturan bireylerden alınan serum örneklerinde, Chlamydia pneumoniae IgG antikorları, ELISA yöntemi ile belirlenmiştir. Ayrıca çalışına grubunu oluşturan bireylere bir anket uygulanarak, kişilere ait yaşam tarzı ve belirli alışkanlıklar ile Chlamydia pneumoniae taşıyıcılığı arasında bir bağlantı olup olmadığı da incelenmiştir. Sonuçlara göre, kontrol bireylerinden 16'sında (%53) pozitif, 14 'ünde (%47) negatif sonuç saptanırken, 39 hasta bireyde (%78) pozitif, 11 'inde de (%22) negatif sonuç tespit edilmiştir. Kontrol ve hasta bireyler arasındaki farklılığın istatistiksel olarak önemli olduğu saptanmıştır (p<0.05).Öğe Akut miyokart infarktüsü için olası iki risk faktörü: Chlamydia pneumoniae taşıyıcılığı ve apolipopotein E polimorfizmi(2003) Ay, Mustafa; Erdem, GünhanSon yıllarda, koroner arterlerde oluşan ve akut miyokart infarktüsü (AMI) patogenezinde önemli rol oynayan aterom plaklarda, Chlamydia pneumoniae türünün varlığı sero-epidemiyolojik araştırmalarla gösterilmiştir. Üzerinde durulan diğer önemli risk faktörlerini ise içinde apolipoprotein E geninin (APOE) de yer aldığı genetik elementler oluşturmaktadır. Bu çalışmanın amacı, AMI geçiren kişilerde C. pneumoniae taşıyıcılığı ile APOE polimorfizmi arasındaki olası bir korelasyonun varlığının araştırılmasıdır. Bireylerden alınan serum örneklerinde, C. pneumoniae IgG antikorları ELISA ile belirlenmiştir. APOE polimorfizmleri, RFLP tekniği kullanılarak saptanmıştır. Çalışmadan elde edilen sonuçlara göre, APOE allel tipleri ve C. pneumoniae taşıyıcılığı arasında istatistiksel olarak önemli bir ilişkinin olmadığı görülmüştür.Öğe Antibiotic resistance and virulence genes in Enterococcus species isolated from raw and processed seafood(Springer, 2022) Çardak, Mine; Özmen Toğay, Sine; Ay, Mustafa; Karaalioğlu, Onur; Erol, Özlem; Bağcı, UfukThis study evaluated the antibiotic resistance characteristics and virulence genes of enterococci isolated from raw and processed seafood sold in the Marmara Region, Turkey. In this context, the enterococcal load was determined as between 1.0 and 2.5 log CFU/g in 39 of a total of 397 samples. It was determined that 117 strains isolated from the samples belonged to Enterococcus gallinarum, E. casseliflavus, E. durans, E. faecium, and E. faecalis species. Erythromycin, tetracycline, streptomycin, and gentamicin resistance was observed, whereas the tetM, ermB, aac(6')-aph(2'')-la genes were found in a majority of the isolates. It was also determined that the isolates carried the agg2 and gelE virulence genes. When all these results are evaluated, the presence of these isolates in aquatic products may pose a risk in terms of food safety and public health.Öğe Antimicrobial Activity Potential of Enterococcus spp. Isolated from some Traditional Turkish Cheeses(Kafkas Univ, Veteriner Fakultesi Dergisi, 2016) Ozmen Togay, Sine; Ay, Mustafa; Sandikci Altunatmaz, Sema; Yilmaz Aksu, Filiz; Erol Tinaztepe, Ozlem; Issa, Ghassan; Buyukunal, Serkan KemalEnterococci can produce enterocins which have antimicrobial activity against Gram-positive and also Gram-negative pathogenic, toxigenic and food-spoilage bacteria. The aim of this study was to determine the antimicrobial activity of Enterococcus spp. isolated from traditional Turkish cheeses such as Kashar, Manyas, Sepet, Kelle, Mihalic, Tulum. The isolates were tested against Listeria monocytogenes, Listeria innocua, Listeria ivanovii, Staphylococcus aureus and Enterococcus faecalis and also detected the presence of entA and entB genes of these isolates. Total 66 of enterococcal isolates were obtained from 34 of cheese samples and 25 of these isolates showed antimicrobial activity against tested reference bacteria by using agar spotting method. Also it was determined most of Enterococcus spp. carried enterocin encoding entA and entB genes. We concluded that these isolates or their enterocins may have a potential for food preservation, however they should be evaluated in terms of food safety.Öğe Distribution of antibiotic resistance and the presence of vancomycin-resistance genes (vanA and vanB) in Enterobacteriaceae isolated from the Sea of Marmara, the Canakkale Strait and the Istanbul Strait, Turkey(Walter De Gruyter Gmbh, 2016) Cardak, Mine; Altug, Gulsen; Ay, Mustafa; Erol, OzlemWe investigated the frequency of antibiotic resistance of Enterobacteriaceae and the presence of vancomyc-in-resistance genes in samples taken from the Sea of Marmara, and the Istanbul and Canakkale Straits, Turkey. Different colony -forming bacteria were isolated and identified with the VITEK 2 Compact 30 system. The antibiotic resistance of the isolates was determined by the disc diffusion method. The isolates were tested against amoxicilin, ampicillin, aztreonam, ceftazidime, cefotaxime, cefuroxime, ofloxacin, vancomycin, tetracycline, kanamycin and gentamycin. The presence of vancomyctn-resistance genes (vanA and vanB) was also investigated. The level of Enterobucteriaceue species was higher in the Sea of Marmara than in the Istanbul Strait and the Canakkale Strait. Isolates showing resistance to the greatest number of antibiotics were identified from E. colt isolates. The resistance of the selected bacterial isolates were as follows: kanamycin (82%), vancomycin (78%) and ampicillin (60%). Some intermediately vancomycin-resistant Enterobacteriaceae isolates had the vanA gene. This study provides evidence of widespread bacterial resistance to clinically relevant antibiotics in marine environments. It also contributes to the knowledge on the distribution of antibiotic resistance among Enterobacteriaceae and indicates the importance of control measures in domestic water treatment.Öğe Effects of spontaneous fermentation on Karalahna and Cabernet Sauvignon young red wines: volatile compounds, sensory profiles and identification of autochthonous yeasts(Springer, 2020) Çelebi Uzkuç, Nesrin Merve; Şişli, Burcu; Ay, Mustafa; Özmen Togay, Sine; Karagül Yüceer, Yonca; Bayhan, Aslı; Kırca Toklucu, Ayşegült The purposes of the present study were to evaluate the volatile compounds and sensory characteristics of young red wines produced by spontaneous and inoculated fermentations of Karalahna (KL) and Cabernet Sauvignon (CS) grapes and to identify the yeasts responsible for spontaneous fermentation by molecular methods. A total of 28 volatile compounds in KL wines and 35 compounds in CS wines were identified and quantified by GC-MS. The concentration of higher alcohols and esters differed significantly among spontaneously fermented and inoculated wines. Spontaneous fermentation resulted in greater amount of higher alcohols in KL wines, while inoculated wines had greater amount of higher alcohols in CS wines. Spontaneously fermented KL and CS wines showed greater amounts of esters than inoculated wines. KL wines obtained by spontaneous fermentation had significantly higher scores than inoculated wines based on fruity and green aromas, body and overall impression. Spontaneously fermented CS wines were found significantly higher in fruity and floral aromas than inoculated wines.Öğe Evaluation of some food safety-related characteristics of Enterococcus faecium and Enterococcus faecalis strains isolated from raw fish samples(Refik Saydam National Public Health Agency (RSNPHA), 2019) Karaalioğlu, Onur; Özmen-Toğay, Sine; Ay, Mustafa; Soysal, Gözde; Çardak, Mine; Bağci, Ufuk; Erol-Tinaztepe, ÖzlemObjective: The aim of this study was to evaluate carrying the antibiotic resistance and virulence genes of Enterococcus faecium and E. faecalis strains isolated from raw fish samples and to investigate antibacterial activity potentials. Methods: Sardine, horse mackerel, red mullet and anchovy samples were analyzed by using Kanamycin Azide Aesculine agar for isolation of enterococci and identified at genus level by Gram staining, catalase test, esculine hydrolysis, growth at pH 9.6, bile salt concentration (40%), 10°C and 45°C and species level by using API 20 Strep biochemical test kits. Antibiotic (streptomycin, chloramphenicol, erythromycin, tetracycline, gentamycin and vancomycin) resistance of 33 enterococcal strains were evaluated by using disk diffusion method. PCR were performed for evaluate the virulence genes (agg2, gelE, cylA, cylB, cylM). Antibacterial activity potentials against reference test bacterias (Staphylococcus aureus ATCC 6538, E. coli ATCC 25922, Listeria monocytogenes ATCC 7644, L. innocua ATCC 33090, E. faecalis NCIMB 700584, E. faecium M74) of the isolates were evaluated by using the agar drop methode. Results: All E. faecalis and E. faecium isolates were resistant to streptomycin, whereas 30 (90.9%) isolates to gentamycin and 14 (42.4%) isolates to vancomycin were found high level resistant. For erythromycin 32 (96.7%) of isolates showed intermediate level of resistancy. Other tested antibiotics, chloramphenicol and tetracycline, were found mostly susceptible, 26 (78.8%) and 30 (90.9%) isolates respectively. Multiple antibiotic resistance was also observed in isolates. In addition, gelE and agg2 genes related to pathogenic function were found in some isolates and ß-hemolytic activity was detected in 4 of isolates. However, there is a potential for significant antibacterial activity against test bacteria including S. aureus and L. monocytogenes in some of the isolates. Conclusion: It is thought that E. faecium and E. faecalis isolates may carry a risk for food safety and public health due to antibiotic resistance and virulence gene transmission, but they also have potential for antimicrobial activity in isolates and all these properties should be evaluated on strain specific. © 2019, Refik Saydam National Public Health Agency (RSNPHA).Öğe EVOLUTION OF YEAST BIODIVERSITY AND VOLATILE COMPOUNDS DURING SPONTANEOUS FERMENTATION OF 'KARASAKIZ' GRAPES FROM DIFFERENT REGIONS OVER TWO CONSECUTIVE VINTAGES(Estacao Vitivinicola Nacional, 2023) Celebi-Uzkuc, Nesrin Merve; Ay, Mustafa; Kirca-Toklucu, AysegulSpontaneous fermentation may produce complex and unique wines with geographical designations due to the region-specific yeast flora and variations in must characteristics. The current study focused on the yeast diversity and changes in volatile compounds during the spontaneous fermentation of 'Karasakiz' grapes from three sub-regions of canakkale province, Turkey for two vintages (2019 and 2020). This is the first study on the diversity of autochthonous yeasts during wine fermentation of 'Karasakiz' variety. In the present work, the strains belong to Hanseniaspora uvarum, Starmerella bacillaris, Pichia terricola, Metschnikowia pulcherrima and Zygoascus hellenicus by PCR-RFLP of the ITS region were identified . A total of 272 isolates were identified as S. cerevisiae. Yeast population and diversity changed related to the sub-region and the vintages. However, H. uvarum and S. bacillaris were common denominators of three regions evaluated. H. uvarum was dominant in the early stages of the fermentation, except for Bozcaada 2019 vintage. Bozcaada region also exhibited higher S. cerevisiae strain diversity compared to other regions. 1-Hexanol and 1-octanol gradually decreased during the fermentation of 'Karasakiz' grapes, while the concentration of isoamyl alcohol, phenylethyl alcohol and ester compounds increased by the fermentation but with some fluctuation.Öğe Genotypic and Phenotypic Evaluation of Heavy Metal Resistance of Enterococcal Isolates from Seafood Products for Consumption(ABADER (Adıyaman Bilimsel Arastırmalar Dernegi), 2023) Çardak, Mine; Özmen Toğay, Sine; Ay, MustafaThe aim of this study was to genotypically and phenotypically evaluate the resistance to heavy metal salts of enterococci isolated from fisheries sold in our country's market for food safety. Using concentrations computed as 1000 ppm of seven heavy metal salts, copper (Cu+2), nickel (Ni+2), cadmium (Cd+2), zinc (Zn+2), lead (Pb+2), chromium (Cr+2), and iron (Fe+2) heavy metal resistance (MIK) tests were conducted on each of the enterococci isolates. As a result of the analyses made in this context, resistance to heavy metal salts such as copper, lead, nickel, and cadmium in many isolates and the presence of copper (tcrB) and cadmium (cadA) resistance genes in some isolates were determined. It is considered that the isolates and the fishery products from which these isolates were obtained may carry risks in terms of food safety and public health. In addition, genes encoding heavy metal resistance are also effective in antibiotic resistance. For this reason, it is stated that these microorganisms gain importance not only in applications such as bioremediation and biomining but also in the healthcare sector. © 2023, ABADER (Adıyaman Bilimsel Arastırmalar Dernegi). All rights reserved.Öğe Investigation of antimicrobial activity and entA and entB genes in Enterococcus faecium and Enterococcus faecalis strains isolated from naturally fermented Turkish white cheeses(Korean Society Food Science & Technology-Kosfost, 2016) Özmen Togay, Sine; Ay, Mustafa; Güneşer, Onur; Karagül Yüceer, YoncaIn this research, the antagonistic effects of Enterococcus faecalis and E. faecium bacteria isolated from naturally fermented Turkish white cheeses, produced without starter culture, against Listeria monocytogenes, L. innocua, L. ivanovii, Staphylococcus aureus, and E. faecalis were evaluated. The presence of entA and entB genes was also detected in the isolates, which had antimicrobial activity. Total 71 strains of E. faecalis and 7 strains of E. faecium were tested; 20 of E. faecalis and none of E. faecium strains showed antimicrobial activity against the tested bacteria using agar spot method. Among E. faecalis strains, which had antimicrobial activity, three strains contained both entA and entB genes, two strains carried only entA gene, and five strains had only entB gene. These cheese-sourced enterococcal strains or their enterocins should be considered to be used for food preservation especially in the dairy industry.Öğe Is There Any Correlation Between The Elevated Plasma Levels and Gene Variations of Factor VIII in Turkish Thrombosis Patients?(Sage Publications Inc, 2011) Ay, Mustafa; Dolek, Bilgen; Erdem, Gunhan; Devecioglu, Omer; Gozukirmizi, NerminWe investigated factor VIII (FVIII) gene mutations in 20 thrombosis patients with high level of FVIII and 20 control healthy participants. Blood samples were used for the determination of FVIII levels using static timing analyze (STA) kits. Informed consent forms were collected from all participants. Factor VIII level was 237 +/- 46 IU/dL in patients group; however, it was 122 +/- 38 IU/dL in healthy control participants. Isolated genomic DNAs were screened using 37 pairs of primers covering promoter region and 26 exons of FVIII gene. Single-strand conformation analysis (SSCA) technique was performed for polymorphism/mutation analyses. We observed polymorph patterns in exon 6, exon 13, exon 14F, exon 19, and exon 25 regions. However, we found no evidence of an association between observed single nucleotide polymorphisms and high thrombosis levels. In conclusion, observed exons polymorphisms do not seem to be associated with a venous thromboembolism.Öğe Nikelce Zengin Topraklardan (Çanakkale) İzole Edilen Bakterilerin Bitki Büyüme Teşvik Edici Potansiyellerinin Belirlenmesi(2022) Karakaş, İlke; Ay, Mustafa; Öztürk, Furkan; Kaya, Selen; Doğru, Nurcihan HacıoğluBitki büyümesini teşvikleyici rizobakteriler (PGPR), bitkinin hastalıklara dirençli hale gelmesinde önemli rol oynamaları sebebiyle sürdürülebilir tarım sistemlerine katkı sağlamaktadır. Bu çalışmada, Çanakkale ili Ezine ilçesinin 6 km kuzeyinde bulunan serpantin yamaçlarında yayılan Alyssum pinifolium (Nyar, T.R. Dudley) bitki köklerinden alınan toprak örneklerinden elde edilen bakteri izolatlarının bitki büyüme teşvik edici potansiyelleri araştırılmıştır. Elde edilen 21 izolatın 16S rDNA analizi ile yapılan identifikasyonlarında Bacillus, Priestia ve Brachybacterium cinslerine ait olduğu saptanmıştır. 21 izolatın PGPR olma potansiyellerini ortaya koymak için in vitro şartlarda azot fikse etme, fosfor çözme yetenekleri, indol-3-asetik asit (IAA), ve siderofor üretme kapasiteleri belirlenmiştir. Çalışmada kullanılan tüm izolatların fosfor çözme yeteneği olduğu saptanmıştır. İzolatların fosfor çözme oranları 2.047 µg/mL ile 2.600 µg/mL arasında bulunmuş olup en yüksek değer Bacillus toyonensis NMCC-157’ten elde edilmiştir. 21 izolat için azot fiksasyon özelliği tespit edilememiştir. IAA üretim yeteneği 51.4 µg/mL ile 278.5 µg/mL arasında ölçülmüş ve en yüksek IAA üretimi Brachybacterium nesterenkovii NY-3 tarafından gerçekleştirilmiştir. İzolatların %47.6’sının siderofor üretim yeteneği olduğu belirlenirken en yüksek siderofor üretimi B. toyonensis NMCC-157’ten elde edilmiştir. Araştırma sonuçları, yüksek miktarlarda siderofor, IAA üretimi ve fosfor çözme gibi bitki büyümesini teşvik edici markör özelliklere sahip olan Bacillus cinslerinin ekonomik açıdan değerli kültür bitkilerinin yetiştirilmesinde biyogübre olarak kullanılma potansiyeline sahip olduğunu göstermiştir.Öğe Nuclear and Mitochondrial DNA of Age-Related Cataract Patients Are Susceptible to Oxidative Damage(Taylor & Francis Inc, 2017) Tinaztepe, Ozlem Erol; Ay, Mustafa; Eser, ErayPurpose: Reactive oxygen species caused by oxidative stress are considered as an important risk factor in the pathogenesis of age-related cataract (ARC). In addition, it has been shown that DNA damage has a potential role in the pathogenesis of cataract. In this study, background DNA damage, oxidative stress-induced DNA damage, and repair of nuclear and mitochondrial DNA of peripheral blood mononuclear cells (PBMCs) of ARC patients were investigated.Methods: The study population included 30 age-matched and sex-matched controls with 30 ARC patients aged 50 years and older. Acute oxidative stress was induced by 200 mu M H2O2. The DNA damage was determined using gene-specific quantitative PCR-based assay in DNA extracted from PBMCs, both at basal condition and after (0, 6, and 20 h) acute oxidative stress.Results: Background level of mitochondrial DNA frequency was higher in cataract patients. The present study revealed that, for the first time, both nDNA and mtDNA of cataract patients were sensitive to the oxidative stress in comparison with healthy individuals. It was found that oxidative DNA damage in PBMCs was almost all repaired within 20 h. Also, time-dependent repair of nDNA and mtDNA damage was not different between cataract patients and healthy individuals.Conclusions: Our findings clearly demonstrate that both nDNA and mtDNA in cataract patients are susceptible to oxidative DNA damage and background level of mitochondrial DNA damage was higher. Also, these results suggest that oxidative DNA damage accumulation (especially mtDNA damage) can play a crucial role in pathogenesis of cataract.Öğe Probiotic characteristics of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrum(Springer, 2019) Bagci, Ufuk; Togay, Sine Ozmen; Temiz, Ayhan; Ay, MustafaAs potential probiotic traits of human milk-isolated bacteria have increasingly been recognized, this study aimed to evaluate the probiotic properties of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrum. Among 118 human milk- and colostrum-isolated lactic cocci, only 29 were identified as Enterococcus. Of these, only four Enterococcus faecium isolates exhibited bacteriocigenic activity against several pathogenic Gram-positive bacteria, including Listeria monocytogenes. These isolates exhibited high acid (up to pH 3.0) and bile tolerance (0.5% oxgall) in simulated gastrointestinal conditions, demonstrating their ability to survive through the upper gastrointestinal tract. All of the E. faecium strains were shown to be sensitive to most of the antibiotics including vancomycin, tetracycline, rifampicin, and erythromycin, while they were resistant to kanamycin and chloramphenicol. None of the strains showed any virulence (gelE, agg2, clyA, clyB, clyM) and antibiotic resistance genes (vanA, vanB, ermB, tetM, and aac(6')-le-aph(2 '')-la). In addition, all the strains were able to assimilate cholesterol, ranging between 25.2-64.1% and they exhibited variable adherence (19-36%) to Caco-2 cells. Based on the overall results of this in vitro study, four of the E. faecium strains isolated from human milk and colostrum can be considered as promising probiotic candidates; however, further in vivo evaluations are required.Öğe Spontan ve Saf Maya Fermentasyonunun Karalahna ve Cabernet Sauvignon Üzümlerinden Elde Edilen Şarabın Uçucu Bileşik, Fenolik Madde ve Antosiyaninleri Üzerine Etkisi ve Spontan Fermentasyonda Rol Alan Mayaların Tanımlanması(2016) Toklucu Kırca, Ayşegül; Ay, Mustafa; Toğay Özmen, Sine; Bayhan, Aslı; Karagül Yüceer, Yonca; Çelebi, Nesrin Merve; Şişli, Burcu
