Evaluation of some food safety-related characteristics of Enterococcus faecium and Enterococcus faecalis strains isolated from raw fish samples
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Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Refik Saydam National Public Health Agency (RSNPHA)
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: The aim of this study was to evaluate carrying the antibiotic resistance and virulence genes of Enterococcus faecium and E. faecalis strains isolated from raw fish samples and to investigate antibacterial activity potentials. Methods: Sardine, horse mackerel, red mullet and anchovy samples were analyzed by using Kanamycin Azide Aesculine agar for isolation of enterococci and identified at genus level by Gram staining, catalase test, esculine hydrolysis, growth at pH 9.6, bile salt concentration (40%), 10°C and 45°C and species level by using API 20 Strep biochemical test kits. Antibiotic (streptomycin, chloramphenicol, erythromycin, tetracycline, gentamycin and vancomycin) resistance of 33 enterococcal strains were evaluated by using disk diffusion method. PCR were performed for evaluate the virulence genes (agg2, gelE, cylA, cylB, cylM). Antibacterial activity potentials against reference test bacterias (Staphylococcus aureus ATCC 6538, E. coli ATCC 25922, Listeria monocytogenes ATCC 7644, L. innocua ATCC 33090, E. faecalis NCIMB 700584, E. faecium M74) of the isolates were evaluated by using the agar drop methode. Results: All E. faecalis and E. faecium isolates were resistant to streptomycin, whereas 30 (90.9%) isolates to gentamycin and 14 (42.4%) isolates to vancomycin were found high level resistant. For erythromycin 32 (96.7%) of isolates showed intermediate level of resistancy. Other tested antibiotics, chloramphenicol and tetracycline, were found mostly susceptible, 26 (78.8%) and 30 (90.9%) isolates respectively. Multiple antibiotic resistance was also observed in isolates. In addition, gelE and agg2 genes related to pathogenic function were found in some isolates and ß-hemolytic activity was detected in 4 of isolates. However, there is a potential for significant antibacterial activity against test bacteria including S. aureus and L. monocytogenes in some of the isolates. Conclusion: It is thought that E. faecium and E. faecalis isolates may carry a risk for food safety and public health due to antibiotic resistance and virulence gene transmission, but they also have potential for antimicrobial activity in isolates and all these properties should be evaluated on strain specific. © 2019, Refik Saydam National Public Health Agency (RSNPHA).
Açıklama
Anahtar Kelimeler
Antibacterial activity; Antibiotic resistance; Enterococcus; Seafood; Virulence factor
Kaynak
Turk Hijyen ve Deneysel Biyoloji Dergisi
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
76
Sayı
3
