Evaluation of some food safety-related characteristics of Enterococcus faecium and Enterococcus faecalis strains isolated from raw fish samples

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dc.contributor.authorKaraalioğlu, Onur
dc.contributor.authorÖzmen-Toğay, Sine
dc.contributor.authorAy, Mustafa
dc.contributor.authorSoysal, Gözde
dc.contributor.authorÇardak, Mine
dc.contributor.authorBağci, Ufuk
dc.contributor.authorErol-Tinaztepe, Özlem
dc.date.accessioned2025-01-27T19:02:30Z
dc.date.available2025-01-27T19:02:30Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractObjective: The aim of this study was to evaluate carrying the antibiotic resistance and virulence genes of Enterococcus faecium and E. faecalis strains isolated from raw fish samples and to investigate antibacterial activity potentials. Methods: Sardine, horse mackerel, red mullet and anchovy samples were analyzed by using Kanamycin Azide Aesculine agar for isolation of enterococci and identified at genus level by Gram staining, catalase test, esculine hydrolysis, growth at pH 9.6, bile salt concentration (40%), 10°C and 45°C and species level by using API 20 Strep biochemical test kits. Antibiotic (streptomycin, chloramphenicol, erythromycin, tetracycline, gentamycin and vancomycin) resistance of 33 enterococcal strains were evaluated by using disk diffusion method. PCR were performed for evaluate the virulence genes (agg2, gelE, cylA, cylB, cylM). Antibacterial activity potentials against reference test bacterias (Staphylococcus aureus ATCC 6538, E. coli ATCC 25922, Listeria monocytogenes ATCC 7644, L. innocua ATCC 33090, E. faecalis NCIMB 700584, E. faecium M74) of the isolates were evaluated by using the agar drop methode. Results: All E. faecalis and E. faecium isolates were resistant to streptomycin, whereas 30 (90.9%) isolates to gentamycin and 14 (42.4%) isolates to vancomycin were found high level resistant. For erythromycin 32 (96.7%) of isolates showed intermediate level of resistancy. Other tested antibiotics, chloramphenicol and tetracycline, were found mostly susceptible, 26 (78.8%) and 30 (90.9%) isolates respectively. Multiple antibiotic resistance was also observed in isolates. In addition, gelE and agg2 genes related to pathogenic function were found in some isolates and ß-hemolytic activity was detected in 4 of isolates. However, there is a potential for significant antibacterial activity against test bacteria including S. aureus and L. monocytogenes in some of the isolates. Conclusion: It is thought that E. faecium and E. faecalis isolates may carry a risk for food safety and public health due to antibiotic resistance and virulence gene transmission, but they also have potential for antimicrobial activity in isolates and all these properties should be evaluated on strain specific. © 2019, Refik Saydam National Public Health Agency (RSNPHA).
dc.identifier.doi10.5505/TurkHijyen.2019.97268
dc.identifier.endpage352
dc.identifier.issn0377-9777
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85074605181
dc.identifier.scopusqualityQ4
dc.identifier.startpage341
dc.identifier.urihttps://doi.org/10.5505/TurkHijyen.2019.97268
dc.identifier.urihttps://hdl.handle.net/20.500.12428/13528
dc.identifier.volume76
dc.indekslendigikaynakScopus
dc.language.isotr
dc.publisherRefik Saydam National Public Health Agency (RSNPHA)
dc.relation.ispartofTurk Hijyen ve Deneysel Biyoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250125
dc.subjectAntibacterial activity; Antibiotic resistance; Enterococcus; Seafood; Virulence factor
dc.titleEvaluation of some food safety-related characteristics of Enterococcus faecium and Enterococcus faecalis strains isolated from raw fish samples
dc.title.alternativeÇiğ balik örneklerinden izole edilen Enterococcus faecium ve Enterococcus faecalis suşlarinin gida güvenliği yönünden bazi özelliklerinin değerlendirilmesi
dc.typeArticle

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