Sclerotic effect of bleomycin on the submandibular gland: An experimental model

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dc.authoridMURATLI, Asli/0000-0003-1901-2477
dc.authoridArik, Deniz/0000-0003-0905-2731
dc.contributor.authorGuclu, Oguz
dc.contributor.authorMuratli, Asli
dc.contributor.authorArik, Deniz
dc.contributor.authorTekin, Kazim
dc.contributor.authorErdogan, Halil
dc.contributor.authorDerekoy, Fevzi Sefa
dc.date.accessioned2025-01-27T20:43:50Z
dc.date.available2025-01-27T20:43:50Z
dc.date.issued2013
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractObjectives: To evaluate the sclerotic effect of bleomycin on the submandibular gland histopathologically and assess it as a possible alternative therapy for sialorrhea. Methods: An experimental model was designed and 18 New Zealand white rabbits were used. The rabbits were divided into two groups: a bleomycin group (n = 9) and a sham group (n = 9). The submandibular glands of the bleomycin group were injected with 0.3 ml bleomycin (3 mg/ml) while the sham group received 0.3 ml saline. Four weeks after the procedure, the glands were removed. Histopathological studies including hematoxylin-eosin and Masson's trichrome stain were carried out. The glands were evaluated for tissue inflammation, fibrosis, edema, lipomatosis, atrophy and congestion. To investigate apoptosis, terminal deoxynucleotidyl transferase (TdT)-mediated digoxigenin-11-dUTP nick-end labeling (TUNEL) immunohistochemical staining was used. Results: In the group injected with bleomycin, inflammation (n = 8), edema (n = 4), fibrosis (n = 3), congestion (n = 4) and lipomatosis (n = 7) were observed. In the sham group, only lipomatosis was observed. The TUNEL assay results were 5.06 +/- 1.18 (p < 0.05) for acinar cells and 8.46 +/- 0.82 (p < 0.05) for ductal cells in the bleomycin group. This was significantly different from the results in the sham group. Conclusions: Apoptosis, inflammation, fibrosis, edema, lipomatosis and congestion were observed in the ductal and acinar cells of the bleomycin group. Bleomycin may be an alternative treatment for sialorrhea cases. However, more research is needed. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
dc.description.sponsorshipScientific Research Projects Department of Canakkale Onsekiz Mart University
dc.description.sponsorshipThis study is supported by Scientific Research Projects Department of Canakkale Onsekiz Mart University.
dc.identifier.doi10.1016/j.ijporl.2013.03.013
dc.identifier.endpage946
dc.identifier.issn0165-5876
dc.identifier.issue6
dc.identifier.pmid23548893
dc.identifier.scopus2-s2.0-84877805083
dc.identifier.scopusqualityQ2
dc.identifier.startpage943
dc.identifier.urihttps://doi.org/10.1016/j.ijporl.2013.03.013
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24393
dc.identifier.volume77
dc.identifier.wosWOS:000320479400012
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofInternational Journal of Pediatric Otorhinolaryngology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBleomycin
dc.subjectSalivary gland
dc.subjectSclerosant
dc.subjectSubmandibular
dc.subjectAtrophy
dc.subjectApoptosis
dc.subjectTUNEL
dc.titleSclerotic effect of bleomycin on the submandibular gland: An experimental model
dc.typeArticle

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