A new nonsurgical experimental model for Asherman syndrome created in rats
[ X ]
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aim: Asherman Syndrome (AS) is a partial or complete obstruction of the uterine cavity with adhesions as a result of trauma. In pre-clinical studies, to be able to show the effectiveness of new treatment methods, first of all, the AS model needs to be created in the animals. The aim of this study is to develop a new and effective nonsurgical method for using in AS and intrauterine adhesions modeling, and through this way, to propose a more effective method for researchers in terms of safety and feasibility. Methods: Twelve female Wistar Albino rats were divided into two groups. It was reached to the left uterine horn transvaginally by using pre-prepared pink color (20 gauge) cannula. While 0.2 ml normal saline was applied to the animals in Group I (control group), 0.2 ml (Trichloroacetic acid) TCA was applied to the animals in Group II (experiment group).The right uterine horns of the animals were left without treatment. After three menstrual cycles, the animals were sacrificed and Hematoxylin-Eosin and Masson’s Trichrom stainings were performed and evaluated histopathologically. Inflammation was evaluated in Hematoxylin-Eosin staining and fibrosis was evaluated in Masson’s Trichrom staining. Results: Whereas the uterine sections of the Group I have normal histologic appearance, inflammation and fibrosis were found in the left uterine sections of the Group 2 by histopathological evaluation. Results were statistically significant (p=0.002). Conclusion: The proposed nonsurgical AS modeling method created disease, and this was also revealed by histopathological examinations. Through this way, a new AS model is suggested without surgery, in which the disease is correctly created.
Açıklama
Anahtar Kelimeler
Tıbbi Araştırmalar Deneysel, Kadın Hastalıkları ve Doğum, Sağlık Bilimleri ve Hizmetleri, Cerrahi
Kaynak
Archives of Clinical and Experimental Medicine
WoS Q Değeri
Scopus Q Değeri
Cilt
3
Sayı
3
