Comparative Study of Antioxidant Properties and Total Phenolic Content of the Extracts of Humulus lupulus L. and Quantification of Bioactive Components by LC-MS/MS and GC-MS

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dc.authoridAY, Mehmet/0000-0002-1095-1614
dc.authoridComert Onder, Ferah/0000-0002-4037-1979
dc.contributor.authorOnder, Ferah Comert
dc.contributor.authorAy, Mehmet
dc.contributor.authorSarker, Satyajit D.
dc.date.accessioned2025-01-27T20:51:57Z
dc.date.available2025-01-27T20:51:57Z
dc.date.issued2013
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this research, antioxidant activities of various extracts obtained from Humulus lupulus L. were compared by DPPH, ABTS, FRAP, and CUPRAC assays. The amount of total phenolic components determined by the Folin-Ciocalteu reagent was found to be highest for 25% aqueous ethanol (9079 +/- 187.83 mg Ferulic acid equivalent/100 g extract) and methanol-1 (directly) (8343 +/- 158.39 mg Ferulic acid equivalent/100 g extract) extracts. The n-hexane extract of H. lupulus exhibited the greatest with DPPH (14.95 +/- 0.03 mu g Trolox equivalent/g sample). The highest phenolic content in the ethanolic extract could be the major contributor to its highest CUPRAC activity (3.15 +/- 0.44 mmol Trolox equivalent/g sample). Methanol-2 (n-hexane, acetone, and methanol) and methanol-3 (n-hexane, dichloromethane, ethylacetate, and methanol) extracts, respectively, exhibited the most potent ABTS (7.35 +/- 0.03 mM Trolox equivalent) and FRAP (1.56 +/- 0.35 mmol Fe2+/g sample) activities. Some of the components from the crude extracts were determined by LC-MS/MS and GC-MS analyses. Comparative screening of antioxidant activities of H. lupulus extracts and quantification of some major components by LC-MS/MS, qualitatively analysis of the reported ones which were optimal under negative ion SIM mode and coinjection, are going to be valuable for food and health applications.
dc.description.sponsorshipCanakkale Onsekiz Mart University [BAP 2010/224]
dc.description.sponsorshipWe acknowledge the Canakkale Onsekiz Mart University for financial support (Research University Grant BAP 2010/224).
dc.identifier.doi10.1021/jf4031508
dc.identifier.endpage10506
dc.identifier.issn0021-8561
dc.identifier.issn1520-5118
dc.identifier.issue44
dc.identifier.pmid24079371
dc.identifier.scopus2-s2.0-84887715881
dc.identifier.scopusqualityQ1
dc.identifier.startpage10498
dc.identifier.urihttps://doi.org/10.1021/jf4031508
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25596
dc.identifier.volume61
dc.identifier.wosWOS:000326774400010
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofJournal of Agricultural and Food Chemistry
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectHumulus lupulus
dc.subjecthop cones
dc.subjecttotal phenolic content
dc.subjectDPPH
dc.subjectCUPRAC
dc.subjectABTS
dc.subjectFRAP
dc.subjectGC-MS
dc.subjectLC-MS/MS
dc.subjectLC-MS (SIM mode)
dc.subjectbioactive components
dc.titleComparative Study of Antioxidant Properties and Total Phenolic Content of the Extracts of Humulus lupulus L. and Quantification of Bioactive Components by LC-MS/MS and GC-MS
dc.typeArticle

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