Genetic and nuclear DNA content variation of stevia (Stevia rebaudiana Bertoni) accessions grown in Turkey

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dc.contributor.authorTiryak, Iskender
dc.contributor.authorKaraoglu, Guelnur
dc.contributor.authorTayyar, Semun
dc.date.accessioned2025-01-27T20:56:06Z
dc.date.available2025-01-27T20:56:06Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe objectives of this study were to make morphological and molecular characterization of Turkish stevia accessions. The field data showed significant variations (P < 0.05) for all morphological parameters (plant height, number of main stems per plant, number of secondary stems, fresh and herb yields) exception with number of secondary stems. The primers of MtP5CS, AtNHX1, MtProDH, Mt-Actin MtSOS1 and MtSOS2 genes were used as loci specific DNA markers. All markers were amplified in Stevia genome and provided a mean of 74.16% polymorphism rate. Flow cytometer analysis showed no statistically significant differences (P < 0.16) for 2C DNA content with a mean of 1.62 pg. The UPGMA analysis revealed that Samsun accession was distinctly separated from the others and had the lowest 2C DNA content (1.52 pg). This study first time showed that the stevia accessions grown in Turkey have limited genetic and no nuclear DNA content variations. The results also revealed that gene targeted functional markers associated with salt and drought tolerance have a great potential to be used as DNA markers to determine the genetic variation in plants.
dc.identifier.doi10.9755/ejfa.2022.v34.i6.2872
dc.identifier.endpage472
dc.identifier.issn2079-052X
dc.identifier.issn2079-0538
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85139348399
dc.identifier.scopusqualityQ3
dc.identifier.startpage465
dc.identifier.urihttps://doi.org/10.9755/ejfa.2022.v34.i6.2872
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26305
dc.identifier.volume34
dc.identifier.wosWOS:000869967100001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherUnited Arab Emirates Univ
dc.relation.ispartofEmirates Journal of Food and Agriculture
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectNuclear DNA content
dc.subjectGene-targeted functional markers
dc.subjectGenetic relataionship
dc.subjectSweet herb
dc.titleGenetic and nuclear DNA content variation of stevia (Stevia rebaudiana Bertoni) accessions grown in Turkey
dc.typeArticle

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