Reserve Carbohydrate Metabolism in Crabtree-Negative and - Positive Yeasts at Different Carbon Sources

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dc.contributor.authorGenc, Tulay Turgut
dc.date.accessioned2025-01-27T20:56:02Z
dc.date.available2025-01-27T20:56:02Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBackground: The fermentation of sugars into ethanol even in the presence of oxygen is referred to as the Crabtree effect. The yeast cells displaying Crabtree effect are indicated as Crabtree-positive yeast. Saccharomyces cerevisiae is Crabtree positive and Debatyomyces occidentalis is Crabtree-negative yeast which does not have Crabtree effect. The reserve carbohydrate metabolism is different in Crabtree-positive and Crabtree-negative yeast cells. The present study aimed to determine the trehalose and glycogen accumulation patterns both in Crabtree-positive and Crabtree-negative yeast species. Methods: In this research, trehalose and glycogen contents of S. cerevisiae and D. occidentalis yeast species were examined in a time course manner in three different carbon sources: glucose, galactose and glycerol. Firstly, yeast cells were grown in rich media supplemented with glucose then all washed and switched to fresh cultures including glucose, galactose and glycerol. Results: In S. cerevisiae yeast cells the overnight accumulated trehalose degraded very rapidly after nonfermentable carbon source replenishment, but this took place in a long time, nearly two days, in D. occidentalis yeast cells. However, whenever D. occidentalis yeast cells shifted to glycerol, all the accumulated trehalose degraded within the twelve hours. Glycogen accumulation in D. occidentalis yeast cells is lower than S. cerevisiae yeast cells both in fermentable and non-fermentable carbon sources. Conclusion: Results indicated that glycogen and trehalose accumulation patterns are completely different in D. occidentalis than S. cerevisiae. Crabtree-negative yeast cells generally, prefer to accumulate glycogen instead of trehalose as reserve carbohydrate. But in our research we proved that Crabtree-negative yeast D. occidentalis, accumulates more trehalose than S. cerevisiae yeast cells in non-fermentable carbon sources.
dc.description.sponsorshipCanakkale Onsekiz Mart University the Scientific Research Coordination Unit [2008-44]
dc.description.sponsorshipThis work was supported by Canakkale Onsekiz Mart University the Scientific Research Coordination Unit, Project number: 2008-44.
dc.identifier.endpage51
dc.identifier.issn2310-5380
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85113324473
dc.identifier.scopusqualityQ2
dc.identifier.startpage47
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26284
dc.identifier.volume8
dc.identifier.wosWOS:000593343600007
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCentre Excellence Molecular Biology-Cemb
dc.relation.ispartofAdvancements in Life Sciences
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectTrehalose
dc.subjectGlycogen
dc.subjectDebaryomyces occidentalis
dc.subjectSaccharomyces cerevisiae
dc.subjectCrabtree effect
dc.titleReserve Carbohydrate Metabolism in Crabtree-Negative and - Positive Yeasts at Different Carbon Sources
dc.typeArticle

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