Disposable electrochemical panel immunosensing systems for the simultaneous detection of potential biomarkers of ovarian cancer

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dc.contributor.authorBilgi Kamac, Melike
dc.contributor.authorYilmaz Kabaca, Aysenur
dc.contributor.authorYilmaz Cilcar, Merve
dc.contributor.authorAltun, Muhammed
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2026-02-03T12:03:04Z
dc.date.available2026-02-03T12:03:04Z
dc.date.issued2026
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractRapid, low-cost, practical, disposable electrochemical panel immunosensor systems were developed for the individual and simultaneous determination of anterior gradient-2 protein (AGR2), folate receptor alpha (FOLR1), glycodelin (GLY), and soluble mesothelin-associated protein (SMRP), which are significantly increased in physiological fluids, particularly in ovarian cancer, and are potential biomarkers in the diagnosis of specific cancer types. To reduce the cost of the panel immunosensor system, the electrodes were hand-fabricated (HSPE), and then the HSPE surfaces were modified with gold nanoparticles (AuNPs). Surface analyses (SEM, XPS, FTIR) have confirmed that the sensor was manufactured robustly. The electrochemical characterization and analysis of the immunosensors were performed using electrochemical impedance spectroscopy, cyclic voltammetry, and differential pulse voltammetry methods. Optimal operating conditions (antibody concentration, antigen, and antibody incubation times) were determined for the prepared single immunosensors. Detection limits, linear detection ranges, selectivity, shelf lives, repeatability, and reproducibility of single and panel immunosensors were determined. The clinical validity of the multi-platform was confirmed by recoveries of over 95% in human serum samples and by ELISA. In this study, low-cost electrochemical panel systems that enable the simultaneous determination of AGR2, GLY, FOLR1, and SMRP biomarkers with high selectivity and accuracy, a wide linear range (1-500 pg mL(-)(1)), low detection limits, and good reproducibility were produced for the first time.
dc.description.sponsorshipCankimath;rimath
dc.description.sponsorshipKaratekin University
dc.description.sponsorshipOpen access funding provided by the Scientific and Technological Research Council of Turkiye (TUB & Idot;TAK).
dc.identifier.doi10.1007/s00604-025-07820-8
dc.identifier.issn0026-3672
dc.identifier.issn1436-5073
dc.identifier.issue2
dc.identifier.pmid41518408
dc.identifier.scopus2-s2.0-105027088144
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s00604-025-07820-8
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34963
dc.identifier.volume193
dc.identifier.wosWOS:001658180900002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.ispartofMicrochimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260130
dc.subjectPanel immunosensor
dc.subjectOvarian cancer
dc.subjectAGR2
dc.subjectGLY
dc.subjectFOLR1
dc.subjectSMRP
dc.titleDisposable electrochemical panel immunosensing systems for the simultaneous detection of potential biomarkers of ovarian cancer
dc.typeArticle

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