Photoelectrocatalytic oxidation of NADH in a flow injection analysis system using a poly-hematoxylin modified glassy carbon electrode

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dc.authoridGiray Dilgin, Didem/0000-0002-3487-3470
dc.authoridDilgin, Yusuf/0000-0002-2980-6871
dc.contributor.authorDilgin, Didem Giray
dc.contributor.authorGligor, Delia
dc.contributor.authorGokcel, H. Ismet
dc.contributor.authorDursun, Zekerya
dc.contributor.authorDilgin, Yusuf
dc.date.accessioned2025-01-27T20:47:28Z
dc.date.available2025-01-27T20:47:28Z
dc.date.issued2010
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA stable electroactive thin film of poly-hematoxylin (poly-HT) was successfully prepared on a glassy carbon electrode (GCE) surface by recording successive cyclic voltammograms of 0.3 mM HT, in a phosphate buffer solution (pH 7.0) containing 0.1 M NaNO3, in the potential range of -0.5 to +2.0 V vs. Ag/AgCl. The deposition of HT on GCE surface can be explained through the electropolymerization process. This poly-HT modified electrode exhibited a good electrocatalytic activity towards the NADH oxidation in a phosphate buffer solution (pH 7.0), and led to a significant decrease in the overpotential by more than 320 mV compared with the bare GCE. In order to perform the photoelectrocatalytic determination of NADH in a flow injection analysis (FIA) system, a home-made flow electrochemical cell with a suitable transparent window for irradiation of the electrode surface was constructed. Flow rate of carrier solution, transmission tubing length, injection volume and applied potential for the amperometric and photoamperometric FIA studies were optimized as 1.3 mL min(-1),10 cm, 100 mu L and +300 mV vs. Ag/AgCl, respectively. The currents obtained from amperometric and photoamperometric measurements in FIA system at optimum conditions were linearly dependent on the NADH concentration and linear calibration curves were obtained in the range of 1.0 X 10(-7)-1.5 X 10(-4) M and in the range of 1.0 x 10(-7)-2.5 X 10(-4) M NADH, respectively. The relative standard deviation (RH)) of six replicate injections of 6.0 x 10(-5) M NADH was calculated as 2.2% and 4.3% for the amperometric and the photoamperometric method, respectively. The limit of detection was found to be 3.0 x 10(-8) M for the photoamperometric determination of NADH. (C) 2010 Elsevier B.V. All rights reserved.
dc.description.sponsorshipScientific and Technological Research Council of Turkey TUBITAK [107T572]; The National Authority for Scientific Research of Romania ANCS [75CB/18.07.2008]
dc.description.sponsorshipThe authors acknowledge the Scientific and Technological Research Council of Turkey TUBITAK (Project No. 107T572) and The National Authority for Scientific Research of Romania ANCS (Project no. 75CB/18.07.2008) for financial support. The authors also thank to Prof. Dr. Ismet Kaya for his help during the performing of some experiments in his Polymer Research Laboratory and his valuable discussions and comments on electropolymerization mechanism of hematoxylin.
dc.identifier.doi10.1016/j.bios.2010.07.120
dc.identifier.endpage417
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.issue2
dc.identifier.pmid20739173
dc.identifier.scopus2-s2.0-77956962045
dc.identifier.scopusqualityQ1
dc.identifier.startpage411
dc.identifier.urihttps://doi.org/10.1016/j.bios.2010.07.120
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24924
dc.identifier.volume26
dc.identifier.wosWOS:000283804400018
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Advanced Technology
dc.relation.ispartofBiosensors & Bioelectronics
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectPhotoelectrocatalysis
dc.subjectFlow injection analysis
dc.subjectModified electrode
dc.subjectNADH
dc.subjectHematoxylin
dc.titlePhotoelectrocatalytic oxidation of NADH in a flow injection analysis system using a poly-hematoxylin modified glassy carbon electrode
dc.typeArticle

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