The Role of Gi and Gs Proteins in Hypoxic Vasoconstriction of Lamb Isolated Pulmonary Artery Rings

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dc.authoridSılan, Coşkun/0000-0002-8352-6571
dc.contributor.authorErbas, Mete
dc.contributor.authorBalbay, Oner
dc.contributor.authorUzun, Ozge
dc.contributor.authorGulec Balbay, Ege
dc.contributor.authorSılan, Coşkun
dc.date.accessioned2025-01-27T20:54:17Z
dc.date.available2025-01-27T20:54:17Z
dc.date.issued2014
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractHypoxic pulmonary vasoconstriction (HPV) is an intrapulmonary adaptive mechanism that matches alveolar ventilation to perfusion. However during prolonged alveolar hypoxia HPV occurs with many pulmonary diseases. Despite intensive studies, cellular mechanisms of HPV are still not well defined. G proteins are a family of membrane-associated proteins believed to be involved in the transduction of various signals including the regulation of vascular tonus. In this study, we aimed to determine the contribution of G(i) and G(s) proteins in hypoxic vasoconstriction of lamb isolated pulmonary artery rings. Pulmonary arteries were isolated from left lower lobe of freshly slaughtered lamb. Arteries suspended in an organ bath filled with Krebs-Henseleit solution and isometric contraction recorded continuously via an isometric transducer connected to a computerised polygraphy system. The solution aerated with 75% N-2 - 20% O-2 - 5% CO2 (normoxic) and 95% N-2 - 5% CO2 (hypoxic) pO(2) of bathing medium was measured continuosly using an oxygen electrode. Pertussis toxin and cholera toxin were used to investigate the role of G(i) and G(s) proteins. In the present study, we observed that hypoxia had no effect on resting force in large artery rings, but it caused a further contraction (1.7 +/- 0.5 mN/mm(2), n=10) in 3 mu M 5-HT precontracted pulmonary arteries rings. Hypoxic vasoconstriction was inhibited by preincubation with 2 mu g/ml cholera toxins (from 2.6 +/- 0.4 mN/mm(2), to 1.0 +/- 0.4 mN/mm(2), n=6) and potentiated by preincubation with pertussis toxins (2 mu g/ml) (from 0.6 +/- 0.4 mN/mm(2), to 1.7 +/- 0.3 mN/mm(2), n=6). These results indicate that signal transduction mediated by G(i) and G(s) proteins may be an important mechanism in the hypoxic vasoconstriction in lamb isolated large pulmonary arteries.
dc.identifier.doi10.9775/kvfd.2013.10040
dc.identifier.endpage285
dc.identifier.issn1300-6045
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84891654248
dc.identifier.scopusqualityQ3
dc.identifier.startpage279
dc.identifier.urihttps://doi.org/10.9775/kvfd.2013.10040
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26031
dc.identifier.volume20
dc.identifier.wosWOS:000333080000018
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isotr
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisi
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectHypoxia
dc.subjectPulmonary vasoconstriction
dc.subjectCholera toxins
dc.subjectPertussis toxins G-proteins
dc.subjectLamb
dc.titleThe Role of Gi and Gs Proteins in Hypoxic Vasoconstriction of Lamb Isolated Pulmonary Artery Rings
dc.title.alternativeİzole kuzu pulmoner arterlerinde gelişen hipoksik pulmoner vazokonstriksiyonda Gi ve Gs proteinlerinin rolü
dc.typeArticle

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