Probiotic characteristics of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrum

Basit Öğe Kaydı
dc.authoridAY, Mustafa/0000-0002-1765-4858
dc.authorid, Ayhan Temiz/0009-0008-3060-7176
dc.authoridbagci, ufuk/0000-0002-1511-2465
dc.contributor.authorBagci, Ufuk
dc.contributor.authorTogay, Sine Ozmen
dc.contributor.authorTemiz, Ayhan
dc.contributor.authorAy, Mustafa
dc.date.accessioned2025-01-27T20:41:17Z
dc.date.available2025-01-27T20:41:17Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractAs potential probiotic traits of human milk-isolated bacteria have increasingly been recognized, this study aimed to evaluate the probiotic properties of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrum. Among 118 human milk- and colostrum-isolated lactic cocci, only 29 were identified as Enterococcus. Of these, only four Enterococcus faecium isolates exhibited bacteriocigenic activity against several pathogenic Gram-positive bacteria, including Listeria monocytogenes. These isolates exhibited high acid (up to pH 3.0) and bile tolerance (0.5% oxgall) in simulated gastrointestinal conditions, demonstrating their ability to survive through the upper gastrointestinal tract. All of the E. faecium strains were shown to be sensitive to most of the antibiotics including vancomycin, tetracycline, rifampicin, and erythromycin, while they were resistant to kanamycin and chloramphenicol. None of the strains showed any virulence (gelE, agg2, clyA, clyB, clyM) and antibiotic resistance genes (vanA, vanB, ermB, tetM, and aac(6')-le-aph(2 '')-la). In addition, all the strains were able to assimilate cholesterol, ranging between 25.2-64.1% and they exhibited variable adherence (19-36%) to Caco-2 cells. Based on the overall results of this in vitro study, four of the E. faecium strains isolated from human milk and colostrum can be considered as promising probiotic candidates; however, further in vivo evaluations are required.
dc.description.sponsorshipHacettepe University Scientific Research Coordination Unit [4753]
dc.description.sponsorshipAuthors would like to thank the Hacettepe University Scientific Research Coordination Unit (Project number: 4753) for financial support to this research project.
dc.identifier.doi10.1007/s12223-019-00687-2
dc.identifier.endpage750
dc.identifier.issn0015-5632
dc.identifier.issn1874-9356
dc.identifier.issue6
dc.identifier.pmid30739237
dc.identifier.scopus2-s2.0-85061305086
dc.identifier.scopusqualityQ2
dc.identifier.startpage735
dc.identifier.urihttps://doi.org/10.1007/s12223-019-00687-2
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24091
dc.identifier.volume64
dc.identifier.wosWOS:000510846800004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofFolia Microbiologica
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectLactic-Acid Bacteria
dc.subjectAntibiotic-Resistant Enterococci
dc.subjectIn-Vitro
dc.subjectCholesterol Assimilation
dc.subjectAntimicrobial Resistance
dc.subjectGel-Electrophoresis
dc.subjectOncorhynchus-Mykiss
dc.subjectVirulence Genes
dc.subjectRainbow-Trout
dc.subjectBreast-Milk
dc.titleProbiotic characteristics of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrum
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu