Multilocus Sequence Typing of Candidatus Phytoplasma Solani and Detection of RNA Viruses Infecting Cucurbits in the Marmara Region, Turkiye

Basit Öğe Kaydı
dc.authorid0000-0002-1366-4389
dc.authorid0000-0002-4503-6344
dc.contributor.authorRanda-Zelyut, Filiz
dc.contributor.authorKaranfil, Ali
dc.date.accessioned2026-02-03T12:00:42Z
dc.date.available2026-02-03T12:00:42Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe stolbur disease associated with Candidatus Phytoplasma solani (CaPsol) is very common in Europe and the Mediterranean regions and adversely affects perennial or annual plants but is rarely reported on cucurbits. We report the first multilocus genetic characterisation of CaPsol strains infecting cucurbit plants and the presence of some RNA viruses prevalent in cucurbits. Between 2019 and 2020, 156 symptomatic and nine asymptomatic plant samples were collected during surveys in cucurbit-growing areas. Symptomatic plants exhibited leaf deformation, growth retardation, yellowing, flower deformation and other virus-like symptoms. Molecular testing detected potyviruses, tobamoviruses and Cucumis melo endornavirus (CmEV) as single and double infections in 108 samples, but CaPsol was determined only in seven plants as a single infection. CaPsol strains infecting squash and watermelon in the Marmara region of T & uuml;rkiye were genetically characterised by describing their tuf, vmp1 and stamp gene sequences. All cucurbit-associated CaPsol strains were of the tuf-b1 genotype and five different stamp genotypes previously reported in other crops and wild plant reservoirs in Europe. Cucurbit CaPsol strains had vmp1 gene sequences corresponding to RFLP types V4 and V14, previously detected in the Mediterranean basin. These strains were not novel cucurbit-specific genotypes but shared similarities with genotypes from diverse plant hosts across Europe. This indicated that CaPsol is not restricted to cucurbits and may persist in alternative plant hosts. Thus, disease management should include monitoring alternative plant reservoirs, rather than focusing solely on cucurbit production areas. Viral infections showed significantly higher prevalence compared to phytoplasma infections in the region.
dc.description.sponsorshipCanakkale Onsekiz Mart Universitesi [FBA-2023-4471]
dc.description.sponsorshipThis work was supported by Canakkale Onsekiz Mart Universitesi, FBA-2023-4471
dc.identifier.doi10.1111/ppa.14138
dc.identifier.endpage1914
dc.identifier.issn0032-0862
dc.identifier.issn1365-3059
dc.identifier.issue6
dc.identifier.scopus2-s2.0-105007632564
dc.identifier.scopusqualityQ1
dc.identifier.startpage1905
dc.identifier.urihttps://doi.org/10.1111/ppa.14138
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34674
dc.identifier.volume74
dc.identifier.wosWOS:001506063200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofPlant Pathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260130
dc.subjectcucurbit
dc.subjectPhytoplasma
dc.subjectstamp gene
dc.subjectstolbur
dc.subjecttuf gene
dc.subjectvmp1 gene
dc.titleMultilocus Sequence Typing of Candidatus Phytoplasma Solani and Detection of RNA Viruses Infecting Cucurbits in the Marmara Region, Turkiye
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu